Evidence map›Paper›PMID 37986910›Full record

ArticlebioRxiv : the preprint server for biology2024

eIF3 engages with 3'-UTR termini of highly translated mRNAs.

Santi Mestre-Fos, Lucas Ferguson, Marena Trinidad, Nicholas T Ingolia, Jamie H D Cate

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Santi Mestre-FosInnovative Genomics Institute, University of California, Berkeley, CA, USA.
Lucas FergusonDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID 0000-0001-5482-4249
Marena TrinidadInnovative Genomics Institute, University of California, Berkeley, CA, USA.ORCID 0000-0001-7839-4642
Nicholas T IngoliaDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.ORCID 0000-0002-3395-1545
Jamie H D CateInnovative Genomics Institute, University of California, Berkeley, CA, USA.ORCID 0000-0001-5965-7902
Innovative Genomics Institute · USHoward Hughes Medical Institute · USQB3 · USUniversity of California, Berkeley · US

Funding

The Role of the Ribosome in the Accuracy of TranslationR01GM065050 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CATE, JAMIE H · 2001 to 2022
$7.4M
Human genetic supplementation without donor DNA or a DNA breakDP1HL156819 · NHLBI · UNIVERSITY OF CALIFORNIA BERKELEY · PI COLLINS, KATHLEEN · 2020 to 2024
$5.8M
Mechanisms of Translation Control in HumansR35GM148352 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI JAMIE H CATE · 2023 to 2026
$2.3M
Diverse and dynamically regulated mRNP composition regulating translationR01GM139008 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI NICHOLAS T INGOLIA · 2023 to 2026
$1.2M
NHLBI NIH HHS DP1 HL156819NIGMS NIH HHS R01 GM065050NIGMS NIH HHS R01 GM139008NIGMS NIH HHS R35 GM148352
6 · The paper itself

Abstract

Stem cell differentiation involves a global increase in protein synthesis to meet the demands of specialized cell types. However, the molecular mechanisms underlying this translational burst and the involvement of initiation factors remains largely unknown. Here, we investigate the role of eukaryotic initiation factor 3 (eIF3) in early differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (NPCs). Using Quick-irCLIP and alternative polyadenylation (APA) Seq, we show eIF3 crosslinks predominantly with 3' untranslated region (3'-UTR) termini of multiple mRNA isoforms, adjacent to the poly(A) tail. Furthermore, we find that eIF3 engagement at 3'-UTR ends is dependent on polyadenylation. High eIF3 crosslinking at 3'-UTR termini of mRNAs correlates with high translational activity, as determined by ribosome profiling, but not with translational efficiency. The results presented here show that eIF3 engages with 3'-UTR termini of highly translated mRNAs, likely reflecting a general rather than specific regulatory function of eIF3, and supporting a role of mRNA circularization in the mechanisms governing mRNA translation.

Identifiers

PMID37986910
PMCPMC10659435
OpenAlexW4388603697

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.