ArticlebioRxiv : the preprint server for biology2023
Engineering water exchange is a safe and effective method for magnetic resonance imaging in diverse cell types.
Austin D C Miller, Soham P Chowdhury, Hadley W Hanson, Sarah K Linderman, Hannah I Ghasemi, Wyatt D Miller, Meghan A Morrissey, Chris D Richardson, Brooke M Gardner, Arnab Mukherjee
Open access · greenAbstract readPreprint
In one paragraphArticle in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed, 1 citations in OpenAlex.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
10 authors at 1 institution in 1 country.
Austin D C MillerBiomolecular Science and Engineering Graduate Program, University of California, Santa Barbara, CA 93106, USA.
Soham P ChowdhuryDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.
Hadley W HansonBiomolecular Science and Engineering Graduate Program, University of California, Santa Barbara, CA 93106, USA.
Sarah K LindermanDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.
Hannah I GhasemiDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.
Wyatt D MillerBiomolecular Science and Engineering Graduate Program, University of California, Santa Barbara, CA 93106, USA.
Meghan A MorrisseyDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.ORCID 0000-0002-0531-4864 Chris D RichardsonDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.ORCID 0000-0003-3122-4851 Brooke M GardnerDepartment of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA 93106, USA.ORCID 0000-0001-7999-8827 Arnab MukherjeeBiomolecular Science and Engineering Graduate Program, University of California, Santa Barbara, CA 93106, USA.
University of California, Santa Barbara · US
Funding
Defining DNA resection and protein localization changes that occur during DSB repairR35GM142975 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI RICHARDSON, CHRIS · 2021 to 2025
$1.9MSignal Integration during PhagocytosisR35GM146935 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI Meghan A Morrissey · 2022 to 2026
$1.9MMetal-free, genetically encoded reporters for calcium recording with MRIR01NS128278 · NINDS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI Tod Edward Kippin, Arnab Mukherjee · 2023 to 2026
$1.9MEngineering fluorescence and magnetic resonance reporter genes for imaging biological function in hypoxic cells and in vivoR35GM133530 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI MUKHERJEE, ARNAB · 2019 to 2023
$1.6MInvestigating the role of AAA+-ATPases in peroxisome biologyR00GM121880 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI GARDNER, BROOKE MEGHAN · 2019 to 2021
$747kA genetically encoded MRI reporter for noninvasive imaging of activity-dependent gene expression in neuronsR03DA050971 · NIDA · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI MUKHERJEE, ARNAB · 2021 to 2021
$218kNIDA NIH HHS R03 DA050971NIGMS NIH HHS R00 GM121880NIGMS NIH HHS R35 GM133530NIGMS NIH HHS R35 GM142975NIGMS NIH HHS R35 GM146935NINDS NIH HHS R01 NS128278
6 · The paper itselfAbstract
Aquaporin-1 (Aqp1), a water channel, has garnered significant interest for cell-based medicine and in vivo synthetic biology due to its ability to be genetically encoded to produce magnetic resonance signals by increasing the rate of water diffusion in cells. However, concerns regarding the effects of Aqp1 overexpression and increased membrane diffusivity on cell physiology have limited its widespread use as a deep-tissue reporter. In this study, we present evidence that Aqp1 generates strong diffusion-based magnetic resonance signals without adversely affecting cell viability or morphology in diverse cell lines derived from mice and humans. Our findings indicate that Aqp1 overexpression does not induce ER stress, which is frequently associated with heterologous expression of membrane proteins. Furthermore, we observed that Aqp1 expression had no detrimental effects on native biological activities, such as phagocytosis, immune response, insulin secretion, and tumor cell migration in the analyzed cell lines. These findings should serve to alleviate any lingering safety concerns regarding the utilization of Aqp1 as a genetic reporter and should foster its broader application as a noninvasive reporter for in vivo studies.
Indexed as
aquaporin-1cell physiologydiffusionMRIreporter geneunfolded protein response
Identifiers
PMID37986852
PMCPMC10659288
OpenAlexW4388621822
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