Evidence map›Paper›PMID 37985890›Full record

ArticleScientific reports2023

LINC01638 sustains human mesenchymal stem cell self-renewal and competency for osteogenic cell fate.

Jonathan A R Gordon, Coralee E Tye, Bodhisattwa Banerjee, Prachi N Ghule, Andre J van Wijnen, Fleur S Kabala, Natalie A Page, Michelle M Falcone, Janet L Stein, Gary S Stein and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.4field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Jonathan A R GordonDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA.
Coralee E TyeDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA.
Bodhisattwa BanerjeeDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA.
Prachi N GhuleDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA.
Andre J van WijnenDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA.
Fleur S KabalaDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA.
Natalie A PageDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA.
Michelle M FalconeDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA.
Janet L SteinDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA.
Gary S SteinDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA.
Jane B LianDepartment of Biochemistry, University of Vermont, Burlington, VT, 05405, USA. jane.lian@uvm.edu.
University of Vermont · US

Funding

Understanding Factors Influencing COVID-19 Testing and Vaccination in Immigrant Low-income and Homeless Populations and Testing Targeted InterventionsU54GM115516 · NIGMS · MAINEHEALTH · PI CLIFFORD JAMES ROSEN · 2017 to 2026
$51.6M
CONTROL OF OSTEOBLAST PROLIFERATION AND DIFFERENTIATIONR01AR039588 · NIAMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI LIAN, JANE B., STEIN, GARY S. · 1990 to 2019
$8.0M
Synthesis of Osteocalcin in BoneR37DE012528 · NIDCR · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI LIAN, JANE B. · 2008 to 2017
$5.0M
Chromatin Organization Regulates OsteogenesisR01DE029311 · NIDCR · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI LIAN, JANE B., STEIN, GARY S. · 2020 to 2024
$2.5M
Upgrade of a FACS Aria Cell SorterS10OD018175 · OD · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI BOYSON, JONATHAN E · 2015 to 2015
$153k
NIAMS NIH HHS R01 AR039588NIDCR NIH HHS R01 DE029311NIDCR NIH HHS R37 DE012528NIGMS NIH HHS U54 GM115516NIH HHS 5R01DE029311NIH HHS R01AR039588NIH HHS S10 OD018175
6 · The paper itself

Abstract

The skeleton forms from multipotent human mesenchymal stem cells (hMSCs) competent to commit to specific lineages. Long noncoding RNAs (lncRNAs) have been identified as key epigenetic regulators of tissue development. However, regulation of osteogenesis by lncRNAs as mediators of commitment to the bone phenotype is largely unexplored. We focused on LINC01638, which is highly expressed in hMSCs and has been studied in cancers, but not in regulating osteogenesis. We demonstrated that LINC01638 promotes initiation of the osteoblast phenotype. Our findings reveal that LINC01638 is present at low levels during the induction of osteoblast differentiation. CRISPRi knockdown of LINC01638 in MSCs prevents osteogenesis and alkaline phosphatase expression, inhibiting osteoblast differentiation. This resulted in decreased MSC growth rate, accompanied by double-strand breaks, DNA damage, and cell senescence. Transcriptome profiling of control and LINC01638-depleted hMSCs identified > 2000 differentially expressed mRNAs related to cell cycle, cell division, spindle formation, DNA repair, and osteogenesis. Using ChIRP-qPCR, molecular mechanisms of chromatin interactions revealed the LINC01638 locus (Chr 22) includes many lncRNAs and bone-related genes. These novel findings identify the obligatory role for LINC01638 to sustain MSC pluripotency regulating osteoblast commitment and growth, as well as for physiological remodeling of bone tissue.

Indexed as

Mesenchymal Stem CellsRNA, Long NoncodingCell DifferentiationCell Self RenewalHumansOsteogenesisRNA, Long Noncoding

Identifiers

PMID37985890
PMCPMC10662126
OpenAlexW4388817478

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.