ArticleNature communications2023
Direct comparison of spatial transcriptional heterogeneity across diverse Bacillus subtilis biofilm communities.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Diversity of biofilm architectures within the genusApplied and environmental microbiology · 2026Article
- Article
- The Bacillus subtilis circadian clock coordinates intricate spatiotemporal organisation.Nature communications · 2026Article
- Article
- Review
- Hosts and Commensal Bacteria Synergistically Antagonize Opportunistic Pathogens at the Single-Cell Resolution.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Microfluidics unveils role of gravity and shear stress on Pseudomonas fluorescens motility and biofilm growth.NPJ biofilms and microbiomes · 2025Article
- The Metabolic and Physiological Responses to Spaceflight of a Lipopeptide-Producing Bacillus subtilis.Microbial biotechnology · 2025Article
- The 2025 motile active matter roadmap.Journal of physics. Condensed matter : an Institute of Physics journal · 2025Review
- Microscopic Analysis of Temperature Effects on Surface Colonization and Biofilm Morphology ofFoods (Basel, Switzerland) · 2025Article
- 3D imaging-driven assembly of multispecies biofilms with antagonistic activity against undesirable bacteria.ISME communications · 2025Article
- Secreted nucleases reclaim extracellular DNA during biofilm development.NPJ biofilms and microbiomes · 2024Article
- Bacillus subtilis EpsA-O: A novel exopolysaccharide structure acting as an efficient adhesive in biofilms.NPJ biofilms and microbiomes · 2024Article
- Insights into the genomic and phenotypic characteristics ofApplied and environmental microbiology · 2024Article
- Deciphering the influence of NaCl on social behaviour ofRoyal Society open science · 2024Article
- Oxidative stress responses in biofilms.Biofilm · 2024Review
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus subtilis can form various types of spatially organised communities on surfaces, such as colonies, pellicles and submerged biofilms. These communities share similarities and differences, and phenotypic heterogeneity has been reported for each type of community. Here, we studied spatial transcriptional heterogeneity across the three types of surface-associated communities. Using RNA-seq analysis of different regions or populations for each community type, we identified genes that are specifically expressed within each selected population. We constructed fluorescent transcriptional fusions for 17 of these genes, and observed their expression in submerged biofilms using time-lapse confocal laser scanning microscopy (CLSM). We found mosaic expression patterns for some genes; in particular, we observed spatially segregated cells displaying opposite regulation of carbon metabolism genes (gapA and gapB), indicative of distinct glycolytic or gluconeogenic regimes coexisting in the same biofilm region. Overall, our study provides a direct comparison of spatial transcriptional heterogeneity, at different scales, for the three main models of B. subtilis surface-associated communities.
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