Evidence map›Paper›PMID 37985202›Full record

ArticleBiotechnology progress

Impact of interstitial flow on cartilage matrix synthesis and NF-kB transcription factor mRNA expression in a novel perfusion bioreactor.

Haneen A Abusharkh, Terreill Robertson, Juana Mendenhall, Bulent A Gozen, Edwin M Tingstad, Nehal I Abu-Lail, David B Thiessen, Bernard J Van Wie

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology progress. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Haneen A AbusharkhVoiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USA.
Terreill RobertsonVoiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USA.
Juana MendenhallDepartment of Chemistry, Morehouse College, Atlanta, Georgia, USA.
Bulent A GozenSchool of Mechanical and Materials Engineering, Washington State University, Pullman, Washington, USA.
Edwin M TingstadInland Orthopedic Surgery and Sports Medicine Clinic, Pullman, Washington, USA.
Nehal I Abu-LailDepartment of Biomedical Engineering and Chemical Engineering, The University of Texas at San Antonio, San Antonio, Texas, USA.
David B ThiessenVoiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USA.
Bernard J Van WieVoiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USA.ORCID 0000-0001-7382-9715
Washington State University · USMorehouse College · USThe University of Texas at San Antonio · US

Funding

TRAINING IN BIOTECHNOLOGY: EMPHASIS ON PROTEIN CHEMISTRYT32GM008336 · NIGMS · WASHINGTON STATE UNIVERSITY · PI CALL, DOUGLAS R. · 1989 to 2023
$8.9M
NIGMS NIH HHS T32 GM008336NIH HHS GM00833631
6 · The paper itself

Abstract

This work is focused on designing an easy-to-use novel perfusion system for articular cartilage (AC) tissue engineering and using it to elucidate the mechanism by which interstitial shear upregulates matrix synthesis by articular chondrocytes (AChs). Porous chitosan-agarose (CHAG) scaffolds were synthesized and compared to bulk agarose (AG) scaffolds. Both scaffolds were seeded with osteoarthritic human AChs and cultured in a novel perfusion system with a medium flow velocity of 0.33 mm/s corresponding to 0.4 mPa surfice shear and 40 mPa CHAG interstitial shear. While there were no statistical differences in cell viability for perfusion versus static cultures for either scaffold type, CHAG scaffolds exhibited a 3.3-fold higher (p < 0.005) cell viability compared to AG scaffold cultures. Effects of combined superficial and interstitial perfusion for CHAG showed 150- and 45-fold (p < 0.0001) increases in total collagen (COL) and 13- and 2.2-fold (p < 0.001) increases in glycosaminoglycans (GAGs) over AG non-perfusion and perfusion cultures, respectively, and a 1.5-fold and 3.6-fold (p < 0.005) increase over non-perfusion CHAG cultures. Contrasting CHAG perfusion and static cultures, chondrogenic gene comparisons showed a 3.5-fold increase in collagen type II/type I (COL2A1/COL1A1) mRNA ratio (p < 0.05), and a 1.3-fold increase in aggrecan mRNA. Observed effects are linked to NF-κB signal transduction pathway inhibition as confirmed by a 3.2-fold (p < 0.05) reduction of NF-κB mRNA expression upon exposure to perfusion. Our results demonstrate that pores play a critical role in improving cell viability and that interstitial flow caused by medium perfusion through the porous scaffolds enhances the expression of chondrogenic genes and extracellular matrix through downregulating NF-κB1.

Indexed as

Cartilage, ArticularChitosanBioreactorsCells, CulturedChondrocytesHumansNF-kappa BPerfusionSepharoseTissue EngineeringTissue ScaffoldsTranscription FactorsChitosanNF-kappa BSepharoseTranscription Factorsarticular cartilageextracellular matrixflow bioreactorinterstitial shearNF-κB

Identifiers

PMID37985202
PMCPMC10922130
OpenAlexW4388869422

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.