Evidence map›Paper›PMID 37982643›Full record

ArticlemSystems2023

scMAR-Seq: a novel workflow for targeted single-cell genomics of microorganisms using radioactive labeling.

Hao-Yu Lo, Konstantin Wink, Henrike Nitz, Matthias Kästner, Detlev Belder, Jochen A Müller, Anne-Kristin Kaster

Abstract read
In one paragraph

Article in mSystems, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Hao-Yu LoInstitute for Biological Interfaces (IBG-5), Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.
Konstantin WinkInstitute for Analytical Chemistry, Leipzig University, Leipzig, Germany.
Henrike NitzDepartment of Environmental Biotechnology, Helmholtz Centre for Environmental Research-UFZ, Leipzig, Germany.
Matthias KästnerDepartment of Environmental Biotechnology, Helmholtz Centre for Environmental Research-UFZ, Leipzig, Germany.
Detlev BelderInstitute for Analytical Chemistry, Leipzig University, Leipzig, Germany.
Jochen A MüllerInstitute for Biological Interfaces (IBG-5), Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.ORCID 0000-0002-0935-1523
Anne-Kristin KasterInstitute for Biological Interfaces (IBG-5), Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, Germany.ORCID 0000-0002-6361-9551

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

importanceA central question in microbial ecology is which member of a community performs a particular metabolism. Several sophisticated isotope labeling techniques are available for analyzing the metabolic function of populations and individual cells in a community. However, these methods are generally either insufficiently sensitive or throughput-limited and thus have limited applicability for the study of complex environmental samples. Here, we present a novel approach that combines highly sensitive radioisotope tracking, microfluidics, high-throughput sorting, and single-cell genomics to simultaneously detect and identify individual microbial cells based solely on their

Indexed as

GenomicsMicrofluidicsWorkflowbenzene degradationFACSmetabolic activitymicroautoradiographymicrobial metabolismmicrofluidicssingle-cell sequencing

Identifiers

PMID37982643
PMCPMC10734494

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.