ArticleAdipocyte2025
Adipogenic characterization of immortalized CD55
Article in Adipocyte, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed, 5 citations in OpenAlex.
- Cytoarchitectural multi-depot profiling reveals immune-metabolic crosstalk in human colon-associated adipose tissue.Cell metabolism · 2026Article
- Epigenetic suppression of creatine kinase B in adipocytes links endoplasmic reticulum stress to obesity-associated inflammation.Molecular metabolism · 2025Article
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMature adipocytes are difficult to study ex vivo, prompting the use of human adipose progenitor cells (hAPCs). However, hAPCs undergo replicative senescence, limiting their utility in long-term studies.
methodsWe inserted human telomerase reverse transcriptase (TERT) into the AAVS1 locus of CD55+ hAPCs derived from abdominal subcutaneous adipose tissue, and characterized the cells before and after adipogenic differentiation.
resultsTERT-hAPCs retained proliferative and adipogenic capacities for over 80 passages, comparable to early-passage wild type hAPCs. Transcriptomic and proteomic analyses confirmed strong adipocyte gene expression. Functionally, TERT-hAPCs responded to insulin and lipolytic stimuli (isoprenaline, dibutyryl cAMP, TNF-α). They adapted well to both 2D and 3D cultures, with improved adipogenesis under spheroid conditions.
conclusionImmortalization of CD55+ hAPCs yields cells with stable proliferative and adipogenic capacity across passages. Being cryopreservable and suitable for both 2D and 3D cultures, TERT-hAPCs offer a reliable, reusable model system for adipocyte studies using cells with a consistent genetic background.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.