Evidence map›Paper›PMID 37982395›Full record

ArticleAnimal biotechnology2024

Condition optimization for electroporation transfection in horse skeletal muscle satellite cells.

Tseweendolmaa Ulaangerel, Minna Yi, Undarmaa Budsuren, Yingchao Shen, Hong Ren, Bold Demuul, Dongyi Bai, Dulguun Dorjgotov, Gantulga Davaakhuu, Tuyatsetseg Jambal and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Tseweendolmaa Ulaangerellnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Minna Yilnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Undarmaa Budsurenlnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Yingchao Shenlnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Hong Renlnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Bold Demuullnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Dongyi Bailnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Dulguun DorjgotovSchool of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar, Mongolia.
Gantulga DavaakhuuInstitute of General and Experimental Biology, Mongolian Academy of Science, Ulaanbaatar, Mongolia.
Tuyatsetseg JambalSchool of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar, Mongolia.
Manglai Dugarjavlnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Gerelchimeg Boulnner Mongolia Key Laboratory of Equine Science Research and Technology Innovation, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
Mongolian University of Science and Technology · MNInner Mongolia Agricultural University · CNMongolian Academy of Sciences · MNMongolian University of Life Sciences · MN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Satellite cells are an important cellular model for studying muscle growth and development and mammalian locomotion-related molecular mechanisms. In this study, we investigated the effects of voltage, pulse duration, and DNA dosage on horse skeletal muscle satellite cells' electroporation transfection efficiency using the eukaryotic expression plasmid Td Tomato-C1 (5.5 kb) encoding the red fluorescent protein gene mainly based on fluorescence-positive cell rate and cell survival rate. By comparison of different voltages, pulse durations, and DNA doses, horse skeletal muscle satellite cells have nearly 80% transfection efficiency under the condition of voltage 120 V, DNA dosage 7 µg/ml, and pulse duration 30 ms. This optimized electroporation condition would facilitate the application of horse skeletal muscle satellite cells in genetic studies of muscle function and related diseases.

Indexed as

Satellite Cells, Skeletal MuscleAnimalsDNAElectroporationHorsesMammalsMuscle, SkeletalPlasmidsTransfectionDNAelectroporation transfectionHorseparameter optimizationsatellite cells

Identifiers

PMID37982395
PMCPMC12674354
OpenAlexW4388833408

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.