ArticleNature communications2023
Diurnal switches in diazotrophic lifestyle increase nitrogen contribution to cereals.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 10 citations in OpenAlex.
- Engineered diazotrophs with host-inducible nitrogen supply systems: Transforming rice farming through innovative nitrogen biofertilizers.Journal of integrative plant biology · 2026Review
- Article
- Non-nodulatingISME communications · 2026Article
- Modification of nifL-nifA operon of Klebsiella oxytoca improves NArchives of microbiology · 2025Article
- Regulation of the Ald gene encoding alanine dehydrogenase and its induction of ammonium-tolerant nitrogen fixation in Paenibacillus polymyxa WLY78.Microbial cell factories · 2025Article
- Enhancing Micronutrient Availability Through Humic Substances and Vermicompost While Growing Artichoke Plants in Calcareous Soil: Insights from a Two-Year Field Study.Plants (Basel, Switzerland) · 2025Article
- NFEmbed: modeling nitrogenase activity via classification and regression with pretrained protein embeddings.Bioinformatics advances · 2025Article
- Optimizing nitrogen use efficiency in forest plantations: mechanistic insights fromForestry research · 2025Review
- Effects of nitrogen application on ammonium assimilation and microenvironment in the rhizosphere of drip-irrigated sunflower under plastic mulch.Frontiers in microbiology · 2024Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uncoupling of biological nitrogen fixation from ammonia assimilation is a prerequisite step for engineering ammonia excretion and improvement of plant-associative nitrogen fixation. In this study, we have identified an amino acid substitution in glutamine synthetase, which provides temperature sensitive biosynthesis of glutamine, the intracellular metabolic signal of the nitrogen status. As a consequence, negative feedback regulation of genes and enzymes subject to nitrogen regulation, including nitrogenase is thermally controlled, enabling ammonia excretion in engineered Escherichia coli and the plant-associated diazotroph Klebsiella oxytoca at 23 °C, but not at 30 °C. We demonstrate that this temperature profile can be exploited to provide diurnal oscillation of ammonia excretion when variant bacteria are used to inoculate cereal crops. We provide evidence that diurnal temperature variation improves nitrogen donation to the plant because the inoculant bacteria have the ability to recover and proliferate at higher temperatures during the daytime.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.