ArticleGenes & genomics2024
Single-cell transcriptomic analysis reveals transcriptional and cell subpopulation differences between human and pig immune cells.
Article in Genes & genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 10 citations in OpenAlex.
- A single-cell profile of the porcine immune repertoire: concurrent profiling of T cell receptor and B cell receptor V(D)J sequences in health.Advanced biotechnology · 2026Article
- Quantum computing-assisted validation of a conserved macrophage suppression module shared by ASFV and PEDV.Genes & genomics · 2026Article
- A single-cell atlas of porcine hematopoietic development.Nature communications · 2026Article
- Why pigs might help us develop better vaccines and drugs against influenza virus.Nature reviews. Immunology · 2026Article
- Review
- Single-Cell Analyses Reveal IKKα Regulates the Interaction Between Macrophages and T Cells in the Doxorubicin-Induced Cardiomyopathy.Inflammation · 2026Article
- Advances in Single-Cell Transcriptomics for Livestock Health.Veterinary sciences · 2026Review
- A single-cell immune atlas of primary and secondary lymphoid organs in pigs.Frontiers in immunology · 2026Article
- Polyphenols ameliorate metabolic disorders by remodeling gut microbiota and regulating Nrf2/NF-κB signaling pathways.Frontiers in microbiology · 2026Review
- Microbiota humanization drives human-like metabolic and immune transcriptomic shifts in pigs.iMetaOmics · 2025Article
- Immunometabolism of Liver Xenotransplantation and Prospective Solutions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Whole-Transcriptome Analysis Reveals the Regulatory Network of Immune Response in Dapulian Pig.Animals : an open access journal from MDPI · 2024Article
- Advances in single-cell transcriptomics in animal research.Journal of animal science and biotechnology · 2024Review
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundThe pig is a promising donor candidate for xenotransplantation. Understanding the differences between human and swine immune systems is critical for addressing xenotransplant rejection and hematopoietic reconstitution. The gene transcriptional profile differences between human and pig immune cell subpopulations have not been studied. To assess the similarities and differences between pigs and humans at the levels of gene transcriptional profiles or cell subpopulations are important for better understanding the cross-species similarity of humans and pigs, and it would help establish the fundamental principles necessary to genetically engineer donor pigs and improve xenotransplantation.
objectiveTo assess the gene transcriptional similarities and differences between pigs and humans.
methodsTwo pigs and two healthy humans' PBMCs were sorted for 10 × genomics single-cell sequence. We generated integrated human-pig scRNA-seq data from human and pig PBMCs and defined the overall gene expression landscape of pig peripheral blood immune cell subpopulations by updating the set of human-porcine homologous genes. The subsets of immune cells were detected by flow cytometry.
resultsThere were significantly less T cells, NK cells and monocytes but more B cells in pig peripheral blood than those in human peripheral blood. High oxidative phosphorylation, HIF-1, glycolysis, and lysosome-related gene expressions in pig CD14
conclusionOur research with integrated scRNA-seq assays identified the different distribution of pig immune cell subpopulations and the different transcriptional profiles of human and pig immune cells. This study enables a deeper understanding of the development and function of porcine immune cells.
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Registered trials
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