P450-catalyzed atom transfer radical cyclization.

Heyu Chen, Wenzhen Fu, Yang Yang

Abstract read

In one paragraph

Article in Methods in enzymology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

3 authors.

Heyu ChenDepartment of Chemistry and Biochemistry, University of California, Santa Barbara, CA, United States.

Wenzhen FuDepartment of Chemistry and Biochemistry, University of California, Santa Barbara, CA, United States.

Yang YangDepartment of Chemistry and Biochemistry, University of California, Santa Barbara, CA, United States; Biomolecular Science and Engineering (BMSE) Program, University of California, Santa Barbara, CA, United States. Electronic address: yang@chem.ucsb.edu.

Funding

An Evolvable Metalloenzyme Platform for Stereoselective Radical BiocatalysisR35GM147387 · NIGMS · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI Yang Yang · 2022 to 2026

$2.0M

NIGMS NIH HHS R35 GM147387

6 · The paper itself

Abstract

Cytochromes P450 have been extensively studied for both fundamental enzymology and biotechnological applications. Over the past decade, by taking inspiration from synthetic organic chemistry, new classes of P450-catalyzed reactions that were not previously encountered in the biological world have been developed to address challenging problems in organic chemistry and asymmetric catalysis. In particular, by repurposing and evolving P450 enzymes, stereoselective biocatalytic atom transfer radical cyclization (ATRC) was developed as a new means to impose stereocontrol over transient free radical intermediates. In this chapter, we describe the detailed experimental protocol for the directed evolution of P450 atom transfer radical cyclases. We also delineate protocols for analytical and preparative scale biocatalytic atom transfer radical cyclization processes. These methods will find application in the development of new P450-catalyzed radical reactions, as well as other synthetically useful processes.

Indexed as

Cytochrome P-450 Enzyme SystemBiocatalysisCatalysisCyclizationMolecular StructureCytochrome P-450 Enzyme SystemAsymmetric catalysisAtom transfer radical cyclizationDirected evolutionP450

Identifiers

PMID37977735

DOI10.1016/bs.mie.2023.09.007

PMCPMC11289761

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.