Evidence map›Paper›PMID 37971563›Full record

ArticleBiotechnology letters2024

Regulation of T16H subcellular localization for promoting its catalytic efficiency in yeast cells.

Jiancong Cao, Bingrun Yang, Mengxia Zhang, Fang Yu

Abstract read
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In one paragraph

Article in Biotechnology letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Jiancong CaoSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Bingrun YangSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Mengxia ZhangSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China.
Fang YuSchool of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, China. yufang@dlpu.edu.cn.ORCID http://orcid.org/0000-0001-7654-1012
Dalian Polytechnic University · CN

Funding

National Natural Science Foundation of China 31970324Science and Technology Basic Resources Investigation Program of China 2019FY100500
6 · The paper itself

Abstract

To investigate the effect of subcellular localization on the transformation efficiency of heterologous expressed functional P450s in yeast. Microbial biotransformation offers a promising substitute for the direct extraction of natural products, but its viability in industrial applications depends on achieving high transformation efficiencies. To investigate the influence of subcellular microenvironments on the activity of heterologously expressed P450s, Catharanthus roseus tabersonine 16-hydroxylase (T16H) was chosen, and its subcellular localization was regulated by fusing organelle-localization signals. Interestingly, this manipulation had no effect on the gene expression levels of T16H, but resulted in varying conversion rates from tabersonine to 16-hydroxy tabersonine. Notably, the highest transformation efficiency was observed in yeast cells expressing peroxisome-localized T16H. Given the alkaline pH optimum for P450s, the alkaline peroxisomal lumen could be a suitable compartment for P450s reactions to achieve high transformation efficiency using yeast cells. Different organelle-localization of T16H in yeast cells resulted in varying conversion rates, suggesting that compartmentalizing the expression of target enzymes could be a viable approach to increase transformation efficiency in yeast.

Indexed as

CatharanthusCytochrome P-450 Enzyme SystemPlant ProteinsSaccharomyces cerevisiaeCYP71D12 protein, Catharanthus roseusCytochrome P-450 Enzyme SystemPlant ProteinsBiotransformation efficiencyCatharanthus rosues T16HCytochrome P450sNatural productsSubcellular localization

Identifiers

PMID37971563
OpenAlexW4388726757

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.