Evidence map›Paper›PMID 37970951›Full record

ArticleEinstein (Sao Paulo, Brazil)2023

Tumor necrosis factor α, and agonist and antagonists of cannabinoid receptor type 1 and type 2 alter the immunophenotype of stem cells from human exfoliated deciduous teeth.

Marizia Trevizani, Laís Lopardi Leal, João Vitor Paes Rettore, Gilson Costa Macedo, Caio César de Souza Alves, Sandra Bertelli Ribeiro de Castro, Antônio Márcio Resende do Carmo, Silvioney Augusto da Silva, Carlos Magno da Costa Maranduba, Fernando de Sá Silva

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Einstein (Sao Paulo, Brazil), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 58% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Marizia TrevizaniInstituto de Ciências Biológicas , Universidade Federal de Juiz de Fora , Juiz de Fora , MG , Brazil .ORCID http://orcid.org/0000-0003-1227-9507
Laís Lopardi LealInstituto de Ciências Biológicas , Universidade Federal de Juiz de Fora , Juiz de Fora , MG , Brazil .ORCID http://orcid.org/0000-0001-8144-302X
João Vitor Paes RettoreInstituto de Ciências Biológicas , Universidade Federal de Juiz de Fora , Juiz de Fora , MG , Brazil .ORCID http://orcid.org/0000-0002-8099-5145
Gilson Costa MacedoInstituto de Ciências Biológicas , Universidade Federal de Juiz de Fora , Juiz de Fora , MG , Brazil .ORCID http://orcid.org/0000-0002-7206-1422
Caio César de Souza AlvesFaculdade de Medicina do Mucuri , Universidade Federal dos Vales do Jequitinhonha e Mucuri , Teófilo Otoni , MG , Brazil .ORCID http://orcid.org/0000-0001-9765-8527
Sandra Bertelli Ribeiro de CastroFaculdade de Medicina do Mucuri , Universidade Federal dos Vales do Jequitinhonha e Mucuri , Teófilo Otoni , MG , Brazil .ORCID http://orcid.org/0000-0002-5535-0919
Antônio Márcio Resende do CarmoInstituto de Ciências Biológicas , Universidade Federal de Juiz de Fora , Juiz de Fora , MG , Brazil .ORCID http://orcid.org/0000-0002-0419-9905
Silvioney Augusto da SilvaInstituto de Ciências Biológicas , Universidade Federal de Juiz de Fora , Juiz de Fora , MG , Brazil .ORCID http://orcid.org/0009-0003-3511-2045
Carlos Magno da Costa MarandubaInstituto de Ciências Biológicas , Universidade Federal de Juiz de Fora , Juiz de Fora , MG , Brazil .ORCID http://orcid.org/0000-0001-7327-1934
Fernando de Sá SilvaUniversidade Federal de Juiz de Fora , Governador Valadares , MG , Brazil .ORCID http://orcid.org/0000-0003-0821-5535
Universidade Federal de Juiz de Fora · BRUniversidade Federal dos Vales do Jequitinhonha e Mucuri · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo verify the involvement of the endocannabinoid system in the immunomodulatory profile of stem cells from human exfoliated deciduous teeth, in the presence or absence of TNF-α, and agonist and antagonists of CB1 and CB2.

methodsStem cells from human exfoliated deciduous teeth were cultured in the presence or absence of an agonist, anandamide, and two antagonists, AM251 and SR144528, of CB1 and CB2 receptors, with or without TNF-α stimulation. For analysis of immunomodulation, surface molecules linked to immunomodulation, namely human leukocyte antigen-DR isotype (HLA-DR), and programmed death ligands 1 (PD-L1) and 2 (PD-L2) were measured using flow cytometry.

resultsThe inhibition of endocannabinoid receptors together with the proinflammatory effect of TNF-α resulted in increased HLA-DR expression in stem cells from human exfoliated deciduous teeth, as well as, in these cells acquiring an anti-inflammatory profile by enhancing the expression of PD-L1 and PD-L2.

conclusionStem cells from human exfoliated deciduous teeth respond to the endocannabinoid system and TNF-α by altering key immune response molecules. Inhibition of endocannabinoid receptors and TNF-α led to an increase in HLA-DR, PD-L1, and PD-L2 levels in stem cells from human exfoliated deciduous teeth. This study shows the interaction between mesenchymal stromal cells and the immune and endocannabinoid systems.

Indexed as

B7-H1 AntigenTumor Necrosis Factor-alphaCell DifferentiationCells, CulturedEndocannabinoidsHLA-DR AntigensHumansReceptor, Cannabinoid, CB1Receptor, Cannabinoid, CB2Receptors, CannabinoidStem CellsTooth, DeciduousB7-H1 AntigenCNR1 protein, humanCNR2 protein, humanEndocannabinoidsHLA-DR AntigensReceptor, Cannabinoid, CB1Receptor, Cannabinoid, CB2Receptors, CannabinoidTumor Necrosis Factor-alpha

Identifiers

PMID37970951
PMCPMC10631756
OpenAlexW4388077399

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.