ArticleEndocrinology2023
A Novel Tissue-Specific Insight into Sex Steroid Fluctuations Throughout the Murine Estrous Cycle.
Article in Endocrinology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.
- A Beginner's Guide for Using Bone in Marine Mammal Research.Integrative and comparative biology · 2026Guideline
- Pregnancy damps thermoregulatory rhythms across timescales through neural estrogen signaling in mice.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Sex Hormones & Exercise in the Musculoskeletal System: From Signaling to Structure and Function.Endocrine reviews · 2026Article
- Endometrial Assembloid Model Reveals Endometrial Gland Development Regulation by Estradiol-Driven WNT7B Suppression.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Temporal organization of gonadal and adrenal steroid fluctuations in male mice.Endocrine connections · 2026Article
- Congenital vs adult-onset estrogen deficiency in female mice: physiological effects and implications for therapy.The Journal of endocrinology · 2026Article
- Early sex-related transcriptional differences in CD8Frontiers in immunology · 2026Article
- 14-3-3θ phosphorylation at S232 reduces its interactome and regulates axonal trafficking.Disease models & mechanisms · 2025Article
- Mouse serum facilitates the expansion of the follicular cavity regardless of the estrous cycle phase.Journal of ovarian research · 2025Article
- 11-Oxygenated Androgens Inhibit Brown Adipose Tissue Differentiation.Endocrinology · 2025Article
- Biological Mechanisms Balancing Torpor and Reproduction in Mammals.Endocrinology · 2025Review
- Adipose Tissue Estrogen Receptor-Alpha Overexpression Ameliorates High-Fat Diet-Induced Adipose Tissue Inflammation.Journal of the Endocrine Society · 2025Article
- Chronic Stress Induces Sex-Specific Renal Mitochondrial Dysfunction in Mice.Function (Oxford, England) · 2025Article
- Real-time behavioral monitoring of C57BL/6J mice during reproductive cycle.Frontiers in neuroscience · 2025Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Serum sex steroid levels fluctuate throughout the reproductive cycle. However, the degree to which sex steroid tissue content mimics circulating content is unknown. Understanding the flux and physiological quantity of tissue steroid content is imperative for targeted hormonal therapy development. Utilizing a gold-standard ultrasensitive liquid chromatography-mass spectrometry (LC/MS) method we determined sex steroid (17β-estradiol [E2], testosterone, androstenedione, and progesterone) fluctuations in serum and in 15 tissues throughout the murine estrous cycle (proestrus, estrus, and diestrus I) and in ovariectomized (OVX) mice. We observed dynamic fluctuations in serum and tissue steroid content throughout the estrous cycle with proestrus generally presenting the highest content of E2, testosterone, and androstenedione, and lowest content of progesterone. In general, the trend in circulating steroid content between the stages of the estrous cycle was mimicked in tissue. However, the absolute amounts of steroid levels when normalized to tissue weight were found to be significantly different between the tissues with the serum steroid quantity often being significantly lower than the tissue quantity. Additionally, we found that OVX mice generally displayed a depletion of all steroids in the various tissues assessed, except in the adrenal glands which were determined to be the main site of peripheral E2 production after ovary removal. This investigation provides a comprehensive analysis of steroid content throughout the estrous cycle in a multitude of tissues and serum. We believe this information will help serve as the basis for the development of physiologically relevant, tissue-specific hormonal therapies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.