Evidence map›Paper›PMID 37966608›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Application of Deep Sequencing in Phage Display.

Vincent Van Deuren, Sander Plessers, Rob Lavigne, Johan Robben

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Vincent Van DeurenDepartment of Biochemistry, Molecular and Structural Biology, KU Leuven, Leuven, Belgium.
Sander PlessersDepartment of Biochemistry, Molecular and Structural Biology, KU Leuven, Leuven, Belgium.
Rob LavigneAnimal and Human Health Engineering (A2H), Leuven (Arenberg), KU Leuven, Leuven, Belgium.
Johan RobbenDepartment of Biochemistry, Molecular and Structural Biology, KU Leuven, Leuven, Belgium. Johan.robben@kuleuven.be.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This chapter describes the workflow to implement deep sequencing into standard phage display experiments on protein libraries. By harvesting the power of high throughput of these techniques, it allows for comprehensive analysis of the naïve library and library evolution in response to selection by ligand binding. The mutagenized target region of the protein variants encoded by the phage pool is analyzed by Illumina paired-end sequencing. Sequence data are processed to extract selection-enriched amino acid motifs. In addition, a complementary long-read sequencing approach is proposed enabling the monitoring of display vector stability.

Indexed as

BacteriophagesCell Surface Display TechniquesGene LibraryHigh-Throughput Nucleotide SequencingMutant ProteinsMutant ProteinsEnrichment detectionIllumina sequencingIndel detectionMolecular evolutionNext-generation sequencingOxford Nanopore sequencingPhage display

Identifiers

PMID37966608

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.