ArticleMethods in molecular biology (Clifton, N.J.)2024
Enumeration of Bacteriophages by Plaque Assay.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Extensive library ofMicrobiology spectrum · 2026Article
- Isolation and therapeutic potential of phage vB_EcoM_GXW16 against a drug-resistant avian pathogenic Escherichia coli strain.Poultry science · 2026Article
- Translational Quantitative Proteomic Assay for Bacteriophages: A New Frontier in Phage Pharmaceutical Development.bioRxiv : the preprint server for biology · 2026Article
- Article
- Analysis of the genomes and phylogenetic relationships of lyticMicrobiology resource announcements · 2025Article
- Article
- Phage Cocktail againstInternational journal of molecular and cellular medicine · 2025Article
- Isolation, characterization and functional analysis of a bacteriophage targeting Culex pipiens pallens resistance-associated Aeromonas hydrophila.Parasites & vectors · 2024Article
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Authors and funding
1 author.
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Abstract
Enumeration of bacteriophages by plaque assay requires the mixing of host-specific bacteria with a lytic bacteriophage of interest in a soft agar overlay (top agar) to prevent the spread of viral infection in the medium; the mixture is then spread on a solid bottom agar. An infection of a single lytic phage particle with a bacterium results in the lysis of the host bacterium and the release of new phage progeny. The new phage progeny released from each bacterium will infect/lyse neighboring bacteria to form a "plaque," which is a clear visible area (that can be counted) with the naked eye. If phages are not present in the mixture, the host bacterium will form a "lawn" in the soft agar overlay and grow to a stationary phase.
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