ArticlemicroLife2023
Fungal drops: a novel approach for macro- and microscopic analyses of fungal mycelial growth.
Article in microLife, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
11 citing papers in PubMed.
- Development of mycelium-reinforced dialdehyde cellulose-polyvinyl alcohol composites for emerging high-performance sustainable materials.Synthetic and systems biotechnology · 2026Article
- Methods for evaluating bacterial dispersal on hyphal networks.microLife · 2026Article
- Article
- Article
- Hierarchical Structure of the Program Used by Filamentous Fungi to Navigate in Confining Microenvironments.Biomimetics (Basel, Switzerland) · 2025Article
- Electrical signaling in fungi: past and present challenges.FEMS microbiology reviews · 2025Review
- Assessing the speed of individual bacteria dispersing on mycelial networks.Evolutionary ecology · 2025Article
- Visualizing liquid distribution across hyphal networks with cellular resolution.Biomicrofluidics · 2024Article
- Fabricated devices for performing bacterial-fungal interaction experiments across scales.Frontiers in microbiology · 2024Review
- Host and nonhost bacteria support bacteriophage dissemination along mycelia and abiotic dispersal networks.microLife · 2024Article
- Associated bacterial communities, confrontation studies, and comparative genomics reveal important interactions betweenFrontiers in fungal biology · 2023Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study presents an inexpensive approach for the macro- and microscopic observation of fungal mycelial growth. The 'fungal drops' method allows to investigate the development of a mycelial network in filamentous microorganisms at the colony and hyphal scales. A heterogeneous environment is created by depositing 15-20 µl drops on a hydrophobic surface at a fixed distance. This system is akin to a two-dimensional (2D) soil-like structure in which aqueous-pockets are intermixed with air-filled pores. The fungus (spores or mycelia) is inoculated into one of the drops, from which hyphal growth and exploration take place. Hyphal structures are assessed at different scales using stereoscopic and microscopic imaging. The former allows to evaluate the local response of regions within the colony (modular behaviour), while the latter can be used for fractal dimension analyses to describe the hyphal network architecture. The method was tested with several species to underpin the transferability to multiple species. In addition, two sets of experiments were carried out to demonstrate its use in fungal biology. First, mycelial reorganization of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.