Evidence map›Paper›PMID 37962704›Full record

ArticleMolecular biology reports2023

FishDNAIDs: DNA barcoding as a tool in the development and validation in silico and in vitro of detection systems to four species of Characiformes of commercial importance in the Brazilian Amazon.

Danniel Rocha Bevilaqua, Jacqueline Silva Batista, Adolfo José da Mota, Ana Caroline Viana da Silva, Andreza Mikeyne Silva da Mota, Kyara Martins Formiga, Carlos Edwar de Carvalho Freitas

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In one paragraph

Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 69% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Danniel Rocha BevilaquaInstituto Federal de Educação Ciência e Tecnologia do Amazonas (IFAM), Campus Avançado, Highway Manoel Urbano, km 77 - Morada do Sol, Manacapuru, AM, CEP 69400-000, Brazil. danniel.bevilaqua@ifam.edu.br.ORCID http://orcid.org/0000-0002-6043-7867
Jacqueline Silva BatistaCoordenação de Biodiversidade (COBIO), Laboratório Temático de Biologia Molecular (LTBM), Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva (PPG-GCBEv), Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, AM, Brazil.
Adolfo José da MotaFaculdade de Ciências Agrárias, Departamento de Ciências Fundamentais e Desenvolvimento Agrícola, Universidade Federal do Amazonas (UFAM), Manaus, AM, Brazil.
Ana Caroline Viana da SilvaPrograma de Pós-Graduação em Genética, Conservação e Biologia Evolutiva (PPG-GCBEv), Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, AM, Brazil.
Andreza Mikeyne Silva da MotaPrograma de Pós-Graduação em Biotecnologia e Recursos Naturais - PPG MBT, Universidade Estadual do Amazonas - UEA, Manaus, AM, Brazil.
Kyara Martins FormigaCoordenação de Biodiversidade (COBIO), Laboratório Temático de Biologia Molecular (LTBM), Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva (PPG-GCBEv), Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, AM, Brazil.
Carlos Edwar de Carvalho FreitasPrograma de Pós-Graduação em Genética, Conservação e Biologia Evolutiva (PPG-GCBEv), Instituto Nacional de Pesquisas da Amazônia (INPA), Manaus, AM, Brazil.
Instituto Nacional de Pesquisas da Amazônia · BRUniversidade do Estado do Amazonas · BRInstituto Federal do Amazonas · BR

Funding

Fundação de Amparo à Pesquisa do Estado do Amazonas 062.01350/2018Fundação de Amparo à Pesquisa do Estado do Amazonas RH-INTERIORIZAÇÃO/Call 003/2014
6 · The paper itself

Abstract

backgroundThe COI mitochondrial gene has been chosen as the "DNA barcode in animals" and the large quantity of genetic information in public databanks gives solid support for the use of DNA barcoding as a promising tool for the development of a specific molecular detection system. METHODS AND

resultsThe present study aimed to develop a Specific Molecular Detection System (SMDS: FishDNAIDs) (primers and probe sets) for the following four target species: Prochilodus nigricans, Potamorhina altamazonica, Psectrogaster rutiloides and Triportheus angulatus, in qPCR assays. In silico and in vitro tests (using gDNA) were performed to test these sets. The database generated contained the cytochrome c oxidase subunit I (COI) nucleotide sequence for 183 specimens of Characiformes, distributed in 34 species representing eight families. In silico, primers designed for the target species amplified different species from the same genus, except for P. rutiloides, which amplified only the target species. In the in vitro test, using the SYBRGreen

conclusionsThe primers and probe sets developed can act as powerful tools for detecting the target Characiformes species.

Indexed as

CharaciformesAnimalsBrazilDNADNA Barcoding, TaxonomicDNA PrimersHumansPhylogenyDNADNA PrimersConservationDetectionDNA barcodeMonitoringSustainable development goals

Identifiers

PMID37962704
OpenAlexW4388656149

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.