Evidence map›Paper›PMID 37961518›Full record

ArticlebioRxiv : the preprint server for biology2023

Modular vector assembly enables rapid assessment of emerging CRISPR technologies.

Abby V McGee, Yanjing V Liu, Audrey L Griffith, Zsofia M Szegletes, Bronte Wen, Carolyn Kraus, Nathan W Miller, Ryan J Steger, Berta Escude Velasco, Justin A Bosch and 7 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 3 institutions in 1 country.

Abby V McGeeGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Yanjing V LiuGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Audrey L GriffithGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Zsofia M SzegletesGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Bronte WenGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Carolyn KrausRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Nathan W MillerGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Ryan J StegerGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Berta Escude VelascoGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Justin A BoschDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
Jonathan D ZirinDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
Raghuvir ViswanathaDepartment of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.
Erik J SontheimerRNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA 01605, USA.
Amy GoodaleGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Matthew A GreeneGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Thomas M GreenGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
John G DoenchGenetic Perturbation Platform, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.ORCID 0000-0002-3707-9889
Broad Institute · USHarvard University · USUniversity of Massachusetts Chan Medical School · US

Funding

Functional genomics resources for the Drosophila - TR&D3P41GM132087 · NIGMS · HARVARD MEDICAL SCHOOL · PI PERRIMON, NORBERT · 2019 to 2023
$6.8M
Advanced tools for HCMI model genetic perturbation and metastasis characterizationU01CA250565 · NCI · BROAD INSTITUTE, INC. · PI DOENCH, JOHN GERARD · 2020 to 2022
$2.4M
NCI NIH HHS U01 CA250565NIGMS NIH HHS P41 GM132087
6 · The paper itself

Abstract

The diversity of CRISPR systems, coupled with scientific ingenuity, has led to an explosion of applications; however, to test newly-described innovations in their model systems, researchers typically embark on cumbersome, one-off cloning projects to generate custom reagents that are optimized for their biological questions. Here, we leverage Golden Gate cloning to create the Fragmid toolkit, a modular set of CRISPR cassettes and delivery technologies, along with a web portal, resulting in a combinatorial platform that enables scalable vector assembly within days. We further demonstrate that multiple CRISPR technologies can be assessed in parallel in a pooled screening format using this resource, enabling the rapid optimization of both novel technologies and cellular models. These results establish Fragmid as a robust system for the rapid design of CRISPR vectors, and we anticipate that this assembly approach will be broadly useful for systematic development, comparison, and dissemination of CRISPR technologies.

Identifiers

PMID37961518
PMCPMC10634825
OpenAlexW4388181102

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.