Evidence map›Paper›PMID 37961474›Full record

ArticlebioRxiv : the preprint server for biology2023

Design of efficient artificial enzymes using crystallographically-enhanced conformational sampling.

Rojo V Rakotoharisoa, Behnoush Seifinoferest, Niayesh Zarifi, Jack D M Miller, Joshua M Rodriguez, Michael C Thompson, Roberto A Chica

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Rojo V RakotoharisoaDepartment of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada, K1N 6N5.ORCID 0000-0002-6901-4529
Behnoush SeifinoferestDepartment of Chemistry and Biochemistry, University of California Merced, Merced, California 95343, United States.ORCID 0000-0002-6671-9654
Niayesh ZarifiDepartment of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada, K1N 6N5.ORCID 0000-0003-4748-7082
Jack D M MillerDepartment of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada, K1N 6N5.ORCID 0009-0008-5462-195X
Joshua M RodriguezDepartment of Chemistry and Biochemistry, University of California Merced, Merced, California 95343, United States.ORCID 0000-0002-2338-7522
Michael C ThompsonDepartment of Chemistry and Biochemistry, University of California Merced, Merced, California 95343, United States.ORCID 0000-0002-6099-2027
Roberto A ChicaDepartment of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario, Canada, K1N 6N5.ORCID 0000-0003-3789-9841

Funding

User Training and OutreachP30GM124169 · NIGMS · UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB · PI PAUL David ADAMS, Gregory L Hura · 2017 to 2026
$28.6M
Eliminating Critical Systematic Errors In Structural Biology With Next-Generation SimulationR01GM124149 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HOLTON, JAMES M · 2017 to 2024
$2.5M
NIGMS NIH HHS P30 GM124169NIGMS NIH HHS R01 GM124149
6 · The paper itself

Abstract

The ability to create efficient artificial enzymes for any chemical reaction is of great interest. Here, we describe a computational design method for increasing catalytic efficiency of

Indexed as

biocatalysisDe novo enzyme designdirected evolutionensemble refinementKemp eliminasesX-ray crystallography

Identifiers

PMID37961474
PMCPMC10635043

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.