Evidence map›Paper›PMID 37961396›Full record

ArticleResearch square2023

Label-Free Functional Analysis of Root-Associated Microbes with Dynamic Quantitative Oblique Back-illumination Microscopy.

Caroline Filan, Madison Green, Abigail Diering, Marcus T Cicerone, Lily S Cheung, Joel E Kostka, Francisco E Robles

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Caroline FilanGeorgia Institute of Technology, George W. Woodruff School of Mechanical Engineering, Atlanta, GA, 30318, USA.
Madison GreenGeorgia Institute of Technology, School of Biological Sciences, Atlanta, GA, 30318, USA.
Abigail DieringGeorgia Institute of Technology, School of Chemistry and Biochemistry, Atlanta, GA, 30332, USA.
Marcus T CiceroneGeorgia Institute of Technology, School of Chemistry and Biochemistry, Atlanta, GA, 30332, USA.
Lily S CheungGeorgia Institute of Technology, School of Chemical and Biomolecular Engineering, Atlanta, GA, 30332, USA.
Joel E KostkaGeorgia Institute of Technology, School of Biological Sciences, Atlanta, GA, 30318, USA.
Francisco E RoblesGeorgia Institute of Technology and Emory University, Wallace H. Coulter Department of Biomedical Engineering, Atlanta, GA, 30318, USA.

Funding

Accessible label-free optical microscopy with quantitative molecular and functional contrastR35GM147437 · NIGMS · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI Francisco E Robles · 2022 to 2026
$1.9M
NIGMS NIH HHS R35 GM147437
6 · The paper itself

Abstract

The increasing global demand for food, coupled with concerns about the environmental impact of synthetic fertilizers, underscores the urgency of developing sustainable agricultural practices. Nitrogen-fixing bacteria, known as diazotrophs, offer a potential solution by converting atmospheric nitrogen into bioavailable forms, reducing the reliance on synthetic fertilizers. However, a deeper understanding of their interactions with plants and other microbes is needed. In this study, we introduce a recently developed label-free 3D quantitative phase imaging technology called dynamic quantitative oblique back-illumination microscopy (DqOBM) to assess the dynamic activity of diazotrophs

Identifiers

PMID37961396
PMCPMC10635382

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.