Evidence map›Paper›PMID 37961380›Full record

ArticlebioRxiv : the preprint server for biology2023

Neutrophil Extracellular Traps have DNAzyme activity that drives bactericidal potential.

Ti-Hsuan Ku, Nikhil Ram-Mohan, Elizabeth J Zudock, Ryuichiro Abe, Samuel Yang

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ti-Hsuan KuDepartment of Emergency Medicine, Stanford University, Stanford, CA 94305, United States.
Nikhil Ram-MohanDepartment of Emergency Medicine, Stanford University, Stanford, CA 94305, United States.ORCID 0000-0002-7761-2772
Elizabeth J ZudockDepartment of Emergency Medicine, Stanford University, Stanford, CA 94305, United States.ORCID 0000-0001-9058-7842
Ryuichiro AbeDepartment of Emergency Medicine, Stanford University, Stanford, CA 94305, United States.ORCID 0000-0001-9907-1180
Samuel YangDepartment of Emergency Medicine, Stanford University, Stanford, CA 94305, United States.ORCID 0000-0003-1123-9036
Stanford University · US

Funding

Changing Cultures in Sepsis: Rapid single-cell pathogen identification and antibiotic susceptibility testing directly from whole bloodR01AI153133 · NIAID · STANFORD UNIVERSITY · PI WONG, PAK KIN, YANG, SAMUEL · 2020 to 2024
$3.7M
A "Culture" Shift: Integrated Bacterial Screening and Antibacterial Susceptibility Test on Microfluidic Digital Array for Bloodstream InfectionsR01AI137272 · NIAID · JOHNS HOPKINS UNIVERSITY · PI WANG, TZA-HUEI JEFF, YANG, SAMUEL · 2018 to 2022
$3.6M
Circulating Bacteriophages for the Diagnosis of SepsisR21GM147838 · NIGMS · STANFORD UNIVERSITY · PI BOLLYKY, PAUL L, YANG, SAMUEL · 2022 to 2023
$429k
NIAID NIH HHS R01 AI137272NIAID NIH HHS R01 AI153133NIGMS NIH HHS R21 GM147838
6 · The paper itself

Abstract

The mechanisms of bacterial killing by neutrophil extracellular traps (NETs) are unclear. DNA, the largest component of NETs is believed to merely be a scaffold with minimal antimicrobial activity through the charge of the backbone. Here, we report that NETs DNA is beyond a scaffold and produces hydroxyl free radicals through the spatially concentrated G-quadruplex/hemin DNAzyme complexes, driving bactericidal effects. Immunofluorescence staining showed colocalization of G-quadruplex and hemin in extruded NETs DNA, and Amplex UltraRed assay portrayed its peroxidase activity. Proximity labeling of bacteria revealed localized concentration of radicals resulting from NETs bacterial trapping.

Identifiers

PMID37961380
PMCPMC10634746
OpenAlexW4387939029

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.