Evidence map›Paper›PMID 37961269›Full record

ArticlebioRxiv : the preprint server for biology2023

Universal inverse modelling of point spread functions for SMLM localization and microscope characterization.

Sheng Liu, Jianwei Chen, Jonas Hellgoth, Lucas-Raphael Müller, Boris Ferdman, Christian Karras, Dafei Xiao, Keith A Lidke, Rainer Heintzmann, Yoav Shechtman and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 7 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 7 institutions in 5 countries.

Sheng LiuDepartment of Physics and Astronomy, University of New Mexico, Albuquerque, NM, USA.ORCID 0000-0003-1225-0763
Jianwei ChenDepartment of Biomedical Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Southern University of Science and Technology, Shenzhen, China.
Jonas HellgothEuropean Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg, Germany.ORCID 0000-0003-0758-1680
Lucas-Raphael MüllerEuropean Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg, Germany.
Boris FerdmanDepartment of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.ORCID 0000-0002-4906-6137
Christian KarrasLeibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, 07745, Jena, Germany.
Dafei XiaoDepartment of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.ORCID 0009-0003-7147-6778
Keith A LidkeDepartment of Physics and Astronomy, University of New Mexico, Albuquerque, NM, USA.ORCID 0000-0002-9328-4318
Rainer HeintzmannInstitute of Physical Chemistry and Abbe Center of Photonics, Friedrich-Schiller-University Jena, Jena, Germany.ORCID 0000-0002-4950-1936
Yoav ShechtmanDepartment of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.ORCID 0000-0001-8498-5203
Yiming LiDepartment of Biomedical Engineering, Guangdong Provincial Key Laboratory of Advanced Biomaterials, Southern University of Science and Technology, Shenzhen, China.ORCID 0000-0003-4109-3824
Jonas RiesEuropean Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg, Germany.ORCID 0000-0002-6640-9250
Technion – Israel Institute of Technology · ILEuropean Molecular Biology Laboratory · DELeibniz Institute of Photonic Technology · DEUniversity of New Mexico · USHarbin Institute of Technology · CNSouthern University of Science and Technology · CNUniversity of Vienna · AT

Funding

Adaptive Tracking and Quantum Imaging for Protein-Protein InteractionsR01GM140284 · NIGMS · UNIVERSITY OF NEW MEXICO · PI BECERRA CHAVEZ, FRANCISCO ELOHIM, LIDKE, KEITH A · 2022 to 2025
$1.8M
International Society for Extracellular Vesicles - Education Day & MeetingR13TR001278 · NCATS · JOHNS HOPKINS UNIVERSITY · PI WITWER, KENNETH W · 2015 to 2015
$13k
NCATS NIH HHS R13 TR001278NIGMS NIH HHS R01 GM140284
6 · The paper itself

Abstract

The point spread function (PSF) of a microscope describes the image of a point emitter. Knowing the accurate PSF model is essential for various imaging tasks, including single molecule localization, aberration correction and deconvolution. Here we present uiPSF (universal inverse modelling of Point Spread Functions), a toolbox to infer accurate PSF models from microscopy data, using either image stacks of fluorescent beads or directly images of blinking fluorophores, the raw data in single molecule localization microscopy (SMLM). The resulting PSF model enables accurate 3D super-resolution imaging using SMLM. Additionally, uiPSF can be used to characterize and optimize a microscope system by quantifying the aberrations, including field-dependent aberrations, and resolutions. Our modular framework is applicable to a variety of microscope modalities and the PSF model incorporates system or sample specific characteristics, e.g., the bead size, depth dependent aberrations and transformations among channels. We demonstrate its application in single or multiple channels or large field-of-view SMLM systems, 4Pi-SMLM, and lattice light-sheet microscopes using either bead data or single molecule blinking data.

Identifiers

PMID37961269
PMCPMC10634843
OpenAlexW4387954135

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.