Evidence map›Paper›PMID 37961214›Full record

ArticlebioRxiv : the preprint server for biology2023

A Cre-dependent reporter mouse for quantitative real-time imaging of Protein Kinase A activity dynamics.

Elizabeth I Tilden, Aditi Maduskar, Anna Oldenborg, Bernardo L Sabatini, Yao Chen

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Elizabeth I TildenDepartment of Neuroscience, Washington University in St. Louis, St. Louis, MO, United States.ORCID 0000-0001-6560-3863
Aditi MaduskarDepartment of Neuroscience, Washington University in St. Louis, St. Louis, MO, United States.
Anna OldenborgDepartment of Neuroscience, Washington University in St. Louis, St. Louis, MO, United States.
Bernardo L SabatiniHoward Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, MA, United States.
Yao ChenDepartment of Neuroscience, Washington University in St. Louis, St. Louis, MO, United States.ORCID 0000-0003-1509-6634
Washington University in St. Louis · USHoward Hughes Medical Institute · US

Funding

Spatial, temporal, and context-dependent features of protein kinase A activityR01NS119821 · NINDS · WASHINGTON UNIVERSITY · PI Yao Chen · 2021 to 2026
$2.4M
The role of wake-associated protein kinase A transients in intrinsic plasticity and learning through agingF30AG084271 · NIA · WASHINGTON UNIVERSITY · PI Elizabeth Tilden · 2023 to 2026
$159k
NIA NIH HHS F30 AG084271NINDS NIH HHS R01 NS119821
6 · The paper itself

Abstract

Intracellular signaling dynamics play a crucial role in cell function. Protein kinase A (PKA) is a key signaling molecule that has diverse functions, from regulating metabolism and brain activity to guiding development and cancer progression. We previously developed an optical reporter, FLIM-AKAR, that allows for quantitative imaging of PKA activity via fluorescence lifetime imaging microscopy and photometry. However, using viral infection or electroporation for the delivery of FLIM-AKAR is invasive, cannot easily target sparse or hard-to-transfect/infect cell types, and results in variable expression. Here, we developed a reporter mouse,

Identifiers

PMID37961214
PMCPMC10635033
OpenAlexW4388249190

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.