Evidence map›Paper›PMID 37960020›Full record

ArticlePlants (Basel, Switzerland)2023

Evaluating Tartary Buckwheat Genotypes with High Callus Induction Rates and the Transcriptomic Profiling during Callus Formation.

Haixia Zhao, Xin Li, Xin Xiao, Tao Wang, Lisong Liu, Chenglei Li, Huala Wu, Zhi Shan, Qi Wu

Open access · goldAbstract read
In one paragraph

Article in Plants (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Haixia ZhaoCollege of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.ORCID 0000-0001-7864-906X
Xin LiCollege of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.
Xin XiaoCollege of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.
Tao WangCollege of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.
Lisong LiuCollege of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.
Chenglei LiCollege of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.
Huala WuCollege of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.
Zhi ShanCollege of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.
Qi WuCollege of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an 625014, China.ORCID 0000-0002-6157-0766
Sichuan Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Due to their complex genotypes, low in vitro regeneration rates, and difficulty in obtaining transgenic plants, studies concerning basic biological research and molecular breeding in Tartary buckwheat (TB) are greatly limited. In this study, the hypocotyls of 60 genotypes of TB (TBC1~60) were used as explants. Of these, TBC14 was selected due to a high callus induction rate of 97.78% under dark and a proliferation coefficient (PC) of 28.2 when cultured on MS medium supplemented with 2.0 mg/L of 2,4-D and 1.5 mg/L of 6-BA. Subsequently, the samples of the calli obtained from TBC14 were collected at 0, 10, 20, and 30 d, and their transcriptomes were sequenced where identified. GO enrichment led to the detection of the most significant active gene set, which was the DNA binding transcription factor activity. The DEGs related to the pathways concerning metabolism, the biosynthesis of secondary metabolites, and hormone signal transduction were the most enriched in the KEGG database. The sets of MYB, AP2/ERF, and bHLH TFs exhibited the highest number of DEGs. Using this enrichment analysis, 421 genes encoding TFs, 47 auxin- and cytokinin-related genes, and 6 signal transduction-associated genes were screened that may play significant roles in callus formation (CF) in TB. Furthermore,

Indexed as

callusgenotypeTartary buckwheattranscriptome

Identifiers

PMID37960020
PMCPMC10647830
OpenAlexW4387904083

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.