Evidence map›Paper›PMID 37958601›Full record

ArticleInternational journal of molecular sciences2023

In Vitro Imaging and Molecular Characterization of Ca

Francesca Camera, Eleonora Colantoni, Tomas Garcia-Sanchez, Barbara Benassi, Claudia Consales, Adeline Muscat, Leslie Vallet, Luis M Mir, Franck Andre, Caterina Merla

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.3field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 4 countries.

Francesca CameraDivision of Health Protection Technologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.ORCID 0000-0001-6455-4094
Eleonora ColantoniDivision of Health Protection Technologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.ORCID 0000-0003-1301-2492
Tomas Garcia-SanchezDepartment of Information and Communication Technologies, Pompeu Fabra University, 08002 Barcelona, Spain.
Barbara BenassiDivision of Health Protection Technologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.ORCID 0000-0002-6570-4434
Claudia ConsalesDivision of Health Protection Technologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.ORCID 0000-0002-7805-4493
Adeline MuscatCNRS, Metabolic and Systemic Aspects of the Oncogenesis, (METSY), Institute Gustave Roussy, Paris-Saclay University, 94805 Villejuif, France.
Leslie ValletCNRS, Metabolic and Systemic Aspects of the Oncogenesis, (METSY), Institute Gustave Roussy, Paris-Saclay University, 94805 Villejuif, France.
Luis M MirCNRS, Metabolic and Systemic Aspects of the Oncogenesis, (METSY), Institute Gustave Roussy, Paris-Saclay University, 94805 Villejuif, France.
Franck AndreCNRS, Metabolic and Systemic Aspects of the Oncogenesis, (METSY), Institute Gustave Roussy, Paris-Saclay University, 94805 Villejuif, France.
Caterina MerlaDivision of Health Protection Technologies, Italian National Agency for Energy, New Technologies and Sustainable Economic Development (ENEA), 00123 Rome, Italy.ORCID 0000-0001-8612-9566
National Agency for New Technologies, Energy and Sustainable Economic Development · ITCentre National de la Recherche Scientifique · FRUniversitat Pompeu Fabra · ES

Funding

COST Action BM1309 European network for innovative uses of EMFs in biomedical applications (EMF-MED) Short Term Scientific Mission 1309Project RomeTechnopole, PNRR Mission 4 Component 2 Investment 1.5, funded by European Union - NextGenerationEU. ECS 0000024, - CUP. B83C22002820006
6 · The paper itself

Abstract

In recent years, the application of pulsed electric fields with very short durations (nanoseconds) and extremely high amplitudes (MV/m) has been investigated for novel medical purposes. Various electric protocols have been explored for different objectives, including the utilization of fractionated pulse doses to enhance cell electrosensitization to the uptake of different markers or an increase in apoptosis. This study focused on the use of fluorescence imaging to examine molecular calcium fluxes induced by different fractionated protocols of short electric pulses in neuroblastoma (SH-SY5Y) and mesenchymal stem cells (HaMSCs) that were electroporated using nanosecond pulsed electric fields. In our experimental setup, we did not observe cell electrosensitization in terms of an increase in calcium flux following the administration of fractionated doses of nanosecond pulsed electric fields with respect to the non-fractionated dose. However, we observed the targeted activation of calcium-dependent genes (

Indexed as

CalciumNeuroblastomaApoptosisCalcium, DietaryGenes, fosHumansSignal TransductionCalciumCalcium, Dietarycalciumcell electrosensitizationelectroporationfractionated electric pulse protocolmesenchymal stem cellnanosecond pulsed electric fieldsneuroblastoma cell

Identifiers

PMID37958601
PMCPMC10647260
OpenAlexW4387959366

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.