Evidence map›Paper›PMID 37957587›Full record

ArticleBMC plant biology2023

In vitro induction of tetraploidy and its effects on phenotypic variations in Populus hopeiensis.

Jian Wu, Qing Zhou, Yaru Sang, Yifan Zhao, Bo Kong, Liang Li, Jiahua Du, Lexun Ma, Min Lu, Pingdong Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Jian WuKey Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming, 650224, China.
Qing ZhouState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Yaru SangState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Yifan ZhaoState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Bo KongState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Liang LiState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Jiahua DuState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Lexun MaState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China.
Min LuInstitute of Genetics and Breeding, Inner Mongolia Academy of Forestry, Hohhot, 010010, China.
Pingdong ZhangState Key Laboratory of Efficient Production of Forest Resource, Beijing Forestry University, Beijing, 100083, China. zhangpd@bjfu.edu.cn.
Beijing Forestry University · CNSouthwest Forestry University · CN

Funding

National Science Foundation of China 31570646
6 · The paper itself

Abstract

backgroundArtificial induction of polyploidy is the most common and effective way to improve the biological properties of Populus and develop new varieties of this tree. In this study, in order to confirm and expand earlier findings, we established a protocol using colchicine and based on an efficient shoot regeneration system of leaf blades to induce tetraploidy in vitro in three genotypes from diploid Populus hopeiensis. The stomatal characteristics, leaf blade size, and growth were evaluated for diploids and tetraploids of three genotypes.

resultsWe found that genotype, preculture duration, colchicine concentration, and colchicine exposure time had highly significant effects on the tetraploid induction rate. The optimal protocol for inducing tetraploidy in P. hopeiensis was to preculture leaf blades for 7 days and then treat them for 4 days with 40 mg/L colchicine. The tetraploid induction rates of genotypes BT1, BT3, and BT8 were 21.2, 11.4 and 16.7%, respectively. A total of 136 tetraploids were identified by flow cytometry analysis and somatic chromosome counting. The stomatal length, width, and density of leaf blades significantly differed between diploid and tetraploid plants. Compared with their diploid counterparts, the tetraploids produced larger leaf blades and had a slower growth rate. Our findings further document the modified morphological characteristics of P. hopeiensis following whole-genome duplication (e.g., induced tetraploidy).

conclusionsWe established a protocol for in vitro induction of tetraploidy from diploid leaf blades treated with colchicine, which can be applied to different genotypes of P. hopeiensis.

Indexed as

PopulusTetraploidyBiological Variation, PopulationColchicineDiploidyPolyploidyColchicineColchicinePhenotypic variationPopulus hopeiensisStomataTetraploid

Identifiers

PMID37957587
PMCPMC10641996
OpenAlexW4388634976

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.