ArticleNature methods2024
Peptide sequencing based on host-guest interaction-assisted nanopore sensing.
Article in Nature methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- An engineered nanopore identifies saccharides, amino acids, peptides and ribonucleotides.Nature biotechnology · 2026Article
- Nanopore-enabled time-resolved monitoring of catecholamine-related phenylalanine metabolism.Nature nanotechnology · 2026Article
- A self-assembled peptide forms α-helical nanopores for ultrasensitive biomarker profiling.Nature nanotechnology · 2026Article
- Exopeptidase-assisted nanopore peptide sequence identification.Nature communications · 2026Article
- High-resolution nanopore peptide sensing, profiling and sequence assembly.Nature nanotechnology · 2026Article
- Epx4 Nanopore With Multiple Constrictions for Single-Molecule Identification.Small methods · 2026Article
- Biosensing With Nanofluidics.Exploration (Beijing, China) · 2026Review
- Nanopore Fingerprinting of Neurodegenerative Proteins and Phosphoproteins within a Minute.JACS Au · 2026Article
- Nanopore-based massively parallel sensing for peptide profiling and protein identification.Nature communications · 2026Article
- Review
- A Graphene Field-Effect Transistor-Based Biosensor Platform for the Electrochemical Profiling of Amino Acids.Biosensors · 2026Article
- Expression and Purification of CytK Nanopores.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Enzyme-assisted nanopore sensor for sensitive and multiplexed detection of microRNAs.Journal of nanobiotechnology · 2025Article
- Protein Design Meets Single-Molecule Detection: Towards Programmable Nanopore Sensors.International journal of molecular sciences · 2025Review
- Article
- Nanopore-based enzyme-linked immunosorbent assay for cancer biomarker detection.Nature nanotechnology · 2025Article
- Unwinding process of DNA/RNA quadruplexes by proteins under label-free nanopore monitoring.Nucleic acids research · 2025Article
- Native globular ferritin nanopore sensor.Nature communications · 2025Article
- Nanopore Environmental Analysis.JACS Au · 2025Review
- After 75 Years, an Alternative to Edman Degradation: A Mechanistic and Efficiency Study of a Base-Induced Method for N-Terminal Peptide Sequencing.Journal of the American Chemical Society · 2025Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct protein sequencing technologies with improved sensitivity and throughput are still needed. Here, we propose an alternative method for peptide sequencing based on enzymatic cleavage and host-guest interaction-assisted nanopore sensing. We serendipitously discovered that the identity of any proteinogenic amino acid in a particular position of a phenylalanine-containing peptide could be determined via current blockage during translocation of the peptide through α-hemolysin nanopores in the presence of cucurbit[7]uril. Building upon this, we further present a proof-of-concept demonstration of peptide sequencing by sequentially cleaving off amino acids from C terminus of a peptide with carboxypeptidases, and then determining their identities and sequence with a peptide probe in nanopore. With future optimization, our results point to a different way of nanopore-based protein sequencing.
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