ArticleNature biomedical engineering2023
Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand.
Article in Nature biomedical engineering, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Mechanical stress-mediated immune and inflammatory regulation:Frontiers in medicine · 2025Pooled it
- Mechanical Signaling: Molecular Mechanisms, Biological Functions, Diseases, and Therapeutic Targets.MedComm · 2025Review
- Sensitive Detection of Intercellular Tensile Forces via Cas12a-Assisted Membrane Molecular Probes.Nano letters · 2025Article
- Nanoscale Cellular Traction Force Quantification: CRISPR-Cas12a Supercharged DNA Tension Sensors in Nanoclustered Ligand Patterns.ACS applied materials & interfaces · 2025Article
- Biomechanics in the tumor microenvironment: from biological functions to potential clinical applications.Experimental hematology & oncology · 2025Review
- A portable and sensitive DNA-based electrochemical sensor for detecting piconewton-scale cellular forces.Analytica chimica acta · 2025Article
- Nuclease-Resistant L-DNA Tension Probes Enable Long-Term Force Mapping of Single Cells and Cell Consortia.Angewandte Chemie (International ed. in English) · 2024Article
- Molecular Force Sensors for Biological Application.International journal of molecular sciences · 2024Review
- Electrochemical DNA-based sensors for measuring cell-generated forces.Biosensors & bioelectronics · 2024Article
- A portable electrochemical DNA sensor for sensitive and tunable detection of piconewton-scale cellular forces.bioRxiv : the preprint server for biology · 2024Article
- Molecular Compressive Force Sensor for Mapping Forces at the Cell-Substrate Interface.Journal of the American Chemical Society · 2024Article
- DNA Nanotechnology for Investigating Mechanical Signaling in the Immune System.Angewandte Chemie (International ed. in English) · 2023Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Molecular forces generated by cell receptors are infrequent and transient, and hence difficult to detect. Here we report an assay that leverages the CRISPR-associated protein 12a (Cas12a) to amplify the detection of cellular traction forces generated by as few as 50 adherent cells. The assay involves the immobilization of a DNA duplex modified with a ligand specific for a cell receptor. Traction forces of tens of piconewtons trigger the dehybridization of the duplex, exposing a cryptic Cas12-activating strand that sets off the indiscriminate Cas12-mediated cleavage of a fluorogenic reporter strand. We used the assay to perform hundreds of force measurements using human platelets from a single blood draw to extract individualized dose-response curves and half-maximal inhibitory concentrations for a panel of antiplatelet drugs. For seven patients who had undergone cardiopulmonary bypass, platelet dysfunction strongly correlated with the need for platelet transfusion to limit bleeding. The Cas12a-mediated detection of cellular traction forces may be used to assess cell state, and to screen for genes, cell-adhesion ligands, drugs or metabolites that modulate cell mechanics.
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Registered trials
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