Evidence map›Paper›PMID 37956553›Full record

ArticleJournal of pharmaceutical and biomedical analysis2024

Alkaline surface treatment and time-resolved reading of mn-doped nanocrystal signal transducer for enhanced bioassay sensitivity.

Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A Fox, Patricia Ellison, Robert O Ryan, Paul J Brett, David AuCoin and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of pharmaceutical and biomedical analysis, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Development of Time-Resolved Luminescence Measurement Instruments for Biosensing and Bioimaging - An Overview.Measurement : journal of the International Measurement Confederation · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Bryan LeeDepartment of Electrical and Biomedical Engineering, University of Nevada, Reno, NV, USA; Biomedical Engineering Program, University of Nevada, Reno, NV, USA. Electronic address: bryanlee@unr.edu.
Gita KharalDepartment of Electrical and Biomedical Engineering, University of Nevada, Reno, NV, USA; Biomedical Engineering Program, University of Nevada, Reno, NV, USA.
Benjamin SreenanDepartment of Electrical and Biomedical Engineering, University of Nevada, Reno, NV, USA; Biomedical Engineering Program, University of Nevada, Reno, NV, USA.
Claire LinDepartment of Electrical and Biomedical Engineering, University of Nevada, Reno, NV, USA.
Ruosheng ZengSchool of Physical Science and Technology, Guangxi University, Nanning, China.
Colin A FoxDepartment of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, USA.
Patricia EllisonDepartment of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, USA.
Robert O RyanDepartment of Biochemistry and Molecular Biology, University of Nevada, Reno, NV, USA.
Paul J BrettDepartment of Microbiology and Immunology, University of Nevada, Reno School of Medicine, NV, USA.
David AuCoinDepartment of Microbiology and Immunology, University of Nevada, Reno School of Medicine, NV, USA.
Xiaoshan ZhuDepartment of Electrical and Biomedical Engineering, University of Nevada, Reno, NV, USA; Biomedical Engineering Program, University of Nevada, Reno, NV, USA. Electronic address: xzhu@unr.edu.
University of Nevada, Reno · USGuangxi University · CN

Funding

Time-Gated Fluorescence Lateral Flow Immunoassay for Melioidosis DiagnosisR15GM135855 · NIGMS · UNIVERSITY OF NEVADA RENO · PI ZHU, XIAOSHAN · 2020 to 2020
$426k
NIGMS NIH HHS R15 GM135855
6 · The paper itself

Abstract

Recently, Mn-doped semiconductor nanocrystals (NCs) with high brightness, long lifetimes, and low-energy excitation are emerging for time-resolved luminescence biosensing/imaging. Following our previous work on Mn-doped NCs, in this work we developed poly(styrene-co-maleic anhydride) (PSMA)-encapsulated Mn-doped AgZnInS/ZnS NCs as signal transducers for immunoassay of capsular polysaccharide (CPS), a surface antigen and also a biomarker of Burkholderia pseudomallei which causes a fatal disease called melioidosis. To enhance the assay sensitivity, a surface treatment for PSMA-encapsulated NCs (NC-probes) was performed to promote the presence of carboxyl groups that help conjugate more anti-CPS antibodies to the surface of NC-probes and thus enhance bioassay signals. Meanwhile, time-resolved reading on the luminescence of NC-probes was adopted to minimize the assay background autofluorescence. Both strategies essentially enhance the assay signal-to-background ratio (or equivalently the assay sensitivity) by increasing the signal and decreasing the background, respectively. Through performing and comparing immunoassays with different NC-probes (with and without surface treatment) and different signal reading methods (time-resolved reading and non-time-resolved reading), it was proven that the immunoassay adopting surface-treated NC-probes and time-resolved reading achieved a lower limit-of-detection (LOD) than the ones adopting non-surface-treated NC-probes or non-time-resolved reading. Moreover, the achieved LOD is comparable to the LOD of immunoassay using enzyme horseradish peroxidase as a signal transducer.

Indexed as

NanoparticlesQuantum DotsLimit of DetectionLuminescenceReadingBioconjugationImmunoassayMn-doped AgZnInS/ZnS NCsSurface modificationTime-resolved luminescence

Identifiers

PMID37956553
PMCPMC10841627
OpenAlexW4388343547

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.