Evidence map›Paper›PMID 37956322›Full record

ArticleNucleic acids research2023

Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.

Kevin R Roy, Jason Gabunilas, Dean Neutel, Michelle Ai, Zoe Yeh, Joyce Samson, Guochang Lyu, Guillaume F Chanfreau

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Control of 3' splice site selection inRNA (New York, N.Y.) · 2025
    Article
  4. Article
  5. Article
  6. Control of 3' splice site selection by the yeast splicing factor Fyv6.bioRxiv : the preprint server for biology · 2024
    Article
  7. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Kevin R RoyDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Jason GabunilasDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Dean NeutelDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Michelle AiDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Zoe YehDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Joyce SamsonDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Guochang LyuDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.
Guillaume F ChanfreauDepartment of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA.ORCID 0000-0002-2505-1950
University of California, Los Angeles · US

Funding

The Control of Gene Expression by Eukaryotic RibonucleasesR35GM130370 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Guillaume F Chanfreau · 2019 to 2026
$3.3M
NIGMS NIH HHS GM 130370NIGMS NIH HHS R35 GM130370
6 · The paper itself

Abstract

The fidelity of splice site selection is critical for proper gene expression. In particular, proper recognition of 3'-splice site (3'SS) sequences by the spliceosome is challenging considering the low complexity of the 3'SS consensus sequence YAG. Here, we show that absence of the Prp18p splicing factor results in genome-wide activation of alternative 3'SS in S. cerevisiae, including highly unusual non-YAG sequences. Usage of these non-canonical 3'SS in the absence of Prp18p is enhanced by upstream poly(U) tracts and by their potential to interact with the first intronic nucleoside, allowing them to dock in the spliceosome active site instead of the normal 3'SS. The role of Prp18p in 3'SS fidelity is facilitated by interactions with Slu7p and Prp8p, but cannot be fulfilled by Slu7p, identifying a unique role for Prp18p in 3'SS fidelity. This fidelity function is synergized by the downstream proofreading activity of the Prp22p helicase, but is independent from another late splicing helicase, Prp43p. Our results show that spliceosomes exhibit remarkably relaxed 3'SS sequence usage in the absence of Prp18p and identify a network of spliceosomal interactions centered on Prp18p which are required to promote the fidelity of the recognition of consensus 3'SS sequences.

Indexed as

RNA Splice SitesSaccharomyces cerevisiaeAlternative SplicingRibonucleoprotein, U5 Small NuclearRNA SplicingRNA Splicing FactorsSaccharomyces cerevisiae ProteinsSpliceosomesPRP18 protein, S cerevisiaeRibonucleoprotein, U5 Small NuclearRNA Splice SitesRNA Splicing FactorsSaccharomyces cerevisiae Proteins

Identifiers

PMID37956322
PMCPMC10711555
OpenAlexW4388622123

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.