Evidence map›Paper›PMID 37956285›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2023

In pursuit of degenerative brain disease diagnosis: Dementia biomarkers detected by DNA aptamer-attached portable graphene biosensor.

Tyler Andrew Bodily, Anirudh Ramanathan, Shanhong Wei, Abhijith Karkisaval, Nemil Bhatt, Cynthia Jerez, Md Anzarul Haque, Armando Ramil, Prachi Heda, Yi Wang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Review
  4. Review
  5. Review
  6. Recent Advances in Xenes Based FET for Biosensing Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  7. Review
  8. Article
  9. Overview of Alzheimer's Disease Neuroimaging Initiative and future clinical trials.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2025
    Review
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Tyler Andrew Bodily *Department of Bioengineering, University of California, San Diego, CA 92093.ORCID 0000-0003-4808-9633
Anirudh Ramanathan *Department of Bioengineering, University of California, San Diego, CA 92093.ORCID 0000-0002-7426-3933
Shanhong Wei *State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Abhijith Karkisaval *Department of Mechanical and Aerospace Engineering, University of California, San Diego, CA 92093.ORCID 0000-0002-4029-0490
Nemil BhattMitchell Center for Neurodegenerative Disorders, Department of Neurology, University of Texas Medical Branch, Galveston, TX 77555.
Cynthia JerezMitchell Center for Neurodegenerative Disorders, Department of Neurology, University of Texas Medical Branch, Galveston, TX 77555.ORCID 0009-0002-2678-1127
Md Anzarul HaqueMitchell Center for Neurodegenerative Disorders, Department of Neurology, University of Texas Medical Branch, Galveston, TX 77555.ORCID 0000-0002-4603-6565
Armando RamilDepartment of Bioengineering, University of California, San Diego, CA 92093.ORCID 0000-0003-2959-9566
Prachi HedaDepartment of Bioengineering, University of California, San Diego, CA 92093.
Yi WangState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Sanjeev KumarDepartment of Computer Science, University of Illinois Urbana-Champaign, Champaign, IL 61820.ORCID 0009-0002-0676-7205
Mikayla LeiteDepartment of Bioengineering, University of California, San Diego, CA 92093.ORCID 0009-0009-6420-6618
Tie LiState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Jianlong ZhaoState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Ratnesh LalDepartment of Bioengineering, University of California, San Diego, CA 92093.

Funding

Formation and Propagation of Tau Oligomeric Strains in Alzheimer's DiseaseR01AG054025 · NIA · UNIVERSITY OF TEXAS MED BR GALVESTON · PI Rakez Kayed · 2016 to 2026
$5.8M
Designing array-AFM to study cellular interactions mediating transmission and progression of AD pathophysiology in brainR56AG058069 · NIA · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LAL, RATNESHWAR · 2018 to 2019
$755k
NIA NIH HHS R01 AG054025NIA NIH HHS R56 AG058069
6 · The paper itself

Abstract

Dementia is a brain disease which results in irreversible and progressive loss of cognition and motor activity. Despite global efforts, there is no simple and reliable diagnosis or treatment option. Current diagnosis involves indirect testing of commonly inaccessible biofluids and low-resolution brain imaging. We have developed a portable, wireless readout-based Graphene field-effect transistor (GFET) biosensor platform that can detect viruses, proteins, and small molecules with single-molecule sensitivity and specificity. We report the detection of three important amyloids, namely, Amyloid beta (Aβ), Tau (τ), and α-Synuclein (αS) using DNA aptamer nanoprobes. These amyloids were isolated, purified, and characterized from the autopsied brain tissues of Alzheimer's Disease (AD) and Parkinson's Disease (PD) patients. The limit of detection (LoD) of the sensor is 10 fM, 1-10 pM, 10-100 fM for Aβ, τ, and αS, respectively. Synthetic as well as autopsied brain-derived amyloids showed a statistically significant sensor response with respect to derived thresholds, confirming the ability to define diseased vs. nondiseased states. The detection of each amyloid was specific to their aptamers; Aβ, τ, and αS peptides when tested, respectively, with aptamers nonspecific to them showed statistically insignificant cross-reactivity. Thus, the aptamer-based GFET biosensor has high sensitivity and precision across a range of epidemiologically significant AD and PD variants. This portable diagnostic system would allow at-home and POC testing for neurodegenerative diseases globally.

Indexed as

Alzheimer DiseaseAptamers, NucleotideGraphiteParkinson DiseaseAmyloid beta-PeptidesBiomarkersHumanstau ProteinsAmyloid beta-PeptidesAptamers, NucleotideBiomarkersGraphitetau ProteinsAlzheimer’s diseaseaptamerbiosensordementiagraphene

Identifiers

PMID37956285
PMCPMC10666025

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.