Evidence map›Paper›PMID 37955865›Full record

ArticleChemistryOpen2024

A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media.

Oleksandra Vozniuk, Zdeněk Kejík, Kateřina Veselá, Markéta Skaličková, Petr Novotný, Róbert Hromádka, Jan Hajduch, Pavel Martásek, Milan Jakubek

Open access · goldAbstract read
In one paragraph

Article in ChemistryOpen, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Oleksandra VozniukBIOCEV, First Faculty of Medicine, Charles University, 252 20, Vestec, Czech Republic.
Zdeněk KejíkDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.
Kateřina VeseláDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.ORCID 0000-0002-2908-8101
Markéta SkaličkováDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.
Petr NovotnýDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.
Róbert HromádkaDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.
Jan HajduchDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.
Pavel MartásekDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.
Milan JakubekDepartment of Paediatrics and Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Ke Karlovu 455/2, 128 08, Prague, Czech Republic.ORCID 0000-0001-7323-3903
Charles University · CZ

Funding

Charles University in Prague Prague Progress Q27/LF1Charles University in Prague Progress Q26-38/LF1Charles University in Prague SVV260521Charles University in Prague UNCE 204064Cooperation Program of Charles UniversityEuropean Union - Next Generation EUMinistry of Education, Youth, and Sports of Czech Republic LM2023053 (EATRIS-CZ)Ministry of Health of Czech Republic NU21-08-00407Ministry of Health of Czech Republic NU22-08-00160Ministry of Health of Czech Republic NU22-D-136National Institute for Neurological Research LX22NPO5107Technology Agency of the Czech Republic TN02000109
6 · The paper itself

Abstract

A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3-100 μg/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.

Indexed as

KetoprofenChromatography, High Pressure LiquidIndicators and ReagentsIndicators and ReagentsKetoprofencellular mediumHPLC/UVketoprofen

Identifiers

PMID37955865
PMCPMC10924040
OpenAlexW4388626938

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.