ReviewJournal, genetic engineering & biotechnology2023
Mastering DNA chromatogram analysis in Sanger sequencing for reliable clinical analysis.
Review in Journal, genetic engineering & biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Rapid Exonuclease-Based De Novo Sequencing Strategy for Long Oligonucleotides via Liquid-Phase Ion Trap Coupled With Mass Spectrometry.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Developmental validation of the rapidpuncher, an automated laser-based sampling system that eliminates cross-contamination in the forensic analysis of FTA cards.International journal of legal medicine · 2026Article
- GEAR Genomics: a user-friendly, open-source web platform enabling interactive genomic analysis for molecular biologists.Nucleic acids research · 2026Article
- CRISPR/Cas9-Based Genome Editing: Understanding Differences in DNA Repair Pathways, Profiles, and Outcomes.International journal of molecular sciences · 2026Review
- Protocol for the identification of recent HIV infection in newly diagnosed individuals.STAR protocols · 2026Article
- The Potential of Bioinformatics in Revolutionizing Disease Diagnosis in Sub-Saharan Africa: A Narrative Review.Health science reports · 2026Article
- Bioprospecting of Aerobic Bacteria with Proteolytic Potential Isolated from Animal and Water Sources in the Three Regions of Mainland Ecuador.International journal of molecular sciences · 2026Article
- Development of a qPCR assay for Fasciola spp. identification and a deep amplicon sequencing method for differentiation of fluke species in UK livestock.PLoS neglected tropical diseases · 2026Article
- Application of SNV Detection Methods for Market Control of Food Products from New Genomic Techniques.International journal of molecular sciences · 2026Review
- Identification of reproductive and molecular biomarkers associated with clinical metritis in dromedary camels (Frontiers in veterinary science · 2026Article
- Prevalence, morphology, and molecular characterization of trypanosomes in bulbuls from Thailand.Parasite (Paris, France) · 2026Article
- Identification of a large off-ladder allele of the D21S2055 locus during population genetics analysis.International journal of legal medicine · 2026Article
- First molecular insight intoVeterinary world · 2025Article
- Association Between FABP3 and FABP4 Genes with Changes in Milk Composition and Fatty Acid Profiles in the Native Southern Yellow Cattle Breed.Veterinary sciences · 2025Article
- Association between KISS1 gene and growth traits in two Middle Eastern sheep breeds: the Iranian Karakul and the Iraqi Awassi.Tropical animal health and production · 2025Article
- Detection of Mycobacteria in Arabian camels and antimycobacterial potential of Moringa oleifera.Scientific reports · 2025Article
- Identification and Phylogenetic Analysis ofPathogens (Basel, Switzerland) · 2025Article
- DNA extraction from long-term preserved formalin-fixed paraffin-embedded samples of thymic epithelial tumours - a performance comparison of three commercial kits.Contemporary oncology (Poznan, Poland) · 2025Article
- Nuclear intron sequence analysis and its implications for molecular systematics of Asian zoonotic blood flukes (Current research in parasitology & vector-borne diseases · 2025Article
- Extrachromosomal circular DNA drives dynamic genome plasticity: emerging roles in disease progression and clinical potential.Theranostics · 2025Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSanger dideoxy sequencing is vital in clinical analysis due to its accuracy, ability to analyze genetic markers like SNPs and STRs, capability to generate reliable DNA profiles, and its role in resolving complex clinical cases. The precision and robustness of Sanger sequencing contribute significantly to the scientific basis of clinical investigations. Though the reading of chromatograms seems to be a routine step, many errors conducted in PCR may lead to consequent limitations in the readings of AGCT peaks. These errors are possibly associated with improper DNA amplification and its subsequent interpretation of DNA sequencing files, such as noisy peaks, artifacts, and confusion between double-peak technical errors, heterozygosity, and double infection potentials. Thus, it is not feasible to read nucleic acid sequences without giving serious attention to these technical problems. To ensure the accuracy of DNA sequencing outcomes, it is also imperative to detect and rectify technical challenges that may lead to misinterpretation of the DNA sequence, resulting in errors and incongruities in subsequent analyses. SHORT
conclusionThis overview sheds light on prominent technical concerns that can emerge prior to and during the interpretation of DNA chromatograms in Sanger sequencing, along with offering strategies to address them effectively. The significance of identifying and tackling these technical limitations during the chromatogram analysis is underscored in this review. Recognizing these concerns can aid in enhancing the quality of downstream analyses for Sanger sequencing results, which holds notable improvement in accuracy, reliability, and ability to provide crucial genetic information in clinical analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.