Evidence map›Paper›PMID 37952188›Full record

ArticleFolia microbiologica2024

Recombinase-aided amplification coupled with lateral flow dipstick for efficient and accurate detection of Bombyx mori nucleopolyhedrovirus.

Runpeng Wang, Sheng Xu, Erjun Wei, Ping He, Yiling Zhang, Qiang Wang, Xudong Tang, Zhongyuan Shen

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In one paragraph

Article in Folia microbiologica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Runpeng WangSchool of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Sheng XuSchool of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Erjun WeiSchool of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Ping HeSchool of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Yiling ZhangSchool of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Qiang WangSchool of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Xudong TangSchool of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Zhongyuan ShenSchool of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China. szysri@163.com.ORCID http://orcid.org/0000-0003-1977-9045

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The infection of Bombyx mori nucleopolyhedrovirus (BmNPV) is one of the main causes of economic losses in sericulture. Thus, it is essential to establish rapid and effective method for BmNPV detection. In the present study, we have developed a recombinase-aided amplification (RAA) to amplify the BmNPV genomic DNA at 37 °C within 30 min, and achieved a rapid detection method by coupling with a lateral flow dipstick (LFD). The RAA-LFD method had a satisfactory detection limit of 6 copies/μL of recombinant plasmid pMD19-T-IE1, and BmNPV infection of silkworm can be detected 12 h post-infection. This method was highly specific for BmNPV, and without cross-reactivity to other silkworm pathogens. In contrast to conventional polymerase chain reaction (PCR), the RAA-LFD assay showed higher sensitivity, cost-saving, and especially is apt to on-site detection of BmNPV infection in the sericulture production.

Indexed as

BombyxNucleic Acid Amplification TechniquesNucleopolyhedrovirusesRecombinasesAnimalsDNA, ViralLimit of DetectionSensitivity and SpecificityDNA, ViralRecombinasesBombyx mori nucleopolyhedrovirusLateral flow dipstickRecombinase-aided amplification

Identifiers

PMID37952188

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.