Evidence map›Paper›PMID 37952154›Full record

ArticleCell reports2023

Cochlear organoids reveal transcriptional programs of postnatal hair cell differentiation from supporting cells.

Gurmannat Kalra, Danielle Lenz, Dunia Abdul-Aziz, Craig Hanna, Mahashweta Basu, Brian R Herb, Carlo Colantuoni, Beatrice Milon, Madhurima Saxena, Amol C Shetty and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
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  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Metabolic Profiling of Cochlear Organoids Identifies α-Ketoglutarate and NADAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024
    Article
  10. Article
  11. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 1 country.

Gurmannat KalraInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA; Program in Molecular Medicine, University of Maryland School of Medicine, Baltimore, MD, USA.
Danielle LenzDepartment of Otolaryngology, Harvard Medical School, Boston, MA, USA; Eaton-Peabody Laboratory, Massachusetts Eye and Ear, Boston, MA, USA.
Dunia Abdul-AzizDepartment of Otolaryngology, Harvard Medical School, Boston, MA, USA; Eaton-Peabody Laboratory, Massachusetts Eye and Ear, Boston, MA, USA.
Craig HannaEaton-Peabody Laboratory, Massachusetts Eye and Ear, Boston, MA, USA.
Mahashweta BasuInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.
Brian R HerbInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.
Carlo ColantuoniInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.
Beatrice MilonDepartment of Otorhinolaryngology-Head & Neck Surgery, University of Maryland School of Medicine, Baltimore, MD, USA.
Madhurima SaxenaDepartment of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Department of Medical Oncology, Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, MA, USA.
Amol C ShettyInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.
Ronna HertzanoNational Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, MD, USA.
Ramesh A ShivdasaniDepartment of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; Department of Medical Oncology, Center for Functional Cancer Epigenetics, Dana-Farber Cancer Institute, Boston, MA, USA; Harvard Stem Cell Institute, Cambridge, MA, USA.
Seth A AmentInstitute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA; Department of Otorhinolaryngology-Head & Neck Surgery, University of Maryland School of Medicine, Baltimore, MD, USA; Department of Psychiatry, University of Maryland School of Medicine, Baltimore, MD, USA.
Albert S B EdgeDepartment of Otolaryngology, Harvard Medical School, Boston, MA, USA; Eaton-Peabody Laboratory, Massachusetts Eye and Ear, Boston, MA, USA; Harvard Stem Cell Institute, Cambridge, MA, USA; Program in Speech and Hearing Bioscience and Technology, Harvard Medical School, Boston, MA, USA. Electronic address: albert_edge@meei.harvard.edu.
University of Maryland, Baltimore · USMassachusetts Eye and Ear Infirmary · USBrigham and Women's Hospital · USNational Institutes of Health · US

Funding

Wnt Signaling in Hair Cell Generation from Supporting CellsR01DC014089 · NIDCD · MASSACHUSETTS EYE AND EAR INFIRMARY · PI EDGE, ALBERT · 2016 to 2020
$1.8M
NIDCD NIH HHS R01 DC014089
6 · The paper itself

Abstract

We explore the changes in chromatin accessibility and transcriptional programs for cochlear hair cell differentiation from postmitotic supporting cells using organoids from postnatal cochlea. The organoids contain cells with transcriptional signatures of differentiating vestibular and cochlear hair cells. Construction of trajectories identifies Lgr5+ cells as progenitors for hair cells, and the genomic data reveal gene regulatory networks leading to hair cells. We validate these networks, demonstrating dynamic changes both in expression and predicted binding sites of transcription factors (TFs) during organoid differentiation. We identify known regulators of hair cell development, Atoh1, Pou4f3, and Gfi1, and the analysis predicts the regulatory factors Tcf4, an E-protein and heterodimerization partner of Atoh1, and Ddit3, a CCAAT/enhancer-binding protein (C/EBP) that represses Hes1 and activates transcription of Wnt-signaling-related genes. Deciphering the signals for hair cell regeneration from mammalian cochlear supporting cells reveals candidates for hair cell (HC) regeneration, which is limited in the adult.

Indexed as

Basic Helix-Loop-Helix ProteinsCochleaAnimalsCell DifferentiationMammalsOrganoidsTranscription FactorsBasic Helix-Loop-Helix ProteinsTranscription FactorsATAC sequencingcochleaCP: Stem cell researchgene expression analysissensory hair cellssingle-cell RNA sequencing

Identifiers

PMID37952154
PMCPMC11007545
OpenAlexW4388583261

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.