Immobilization of Horseradish Peroxidase on Ca Alginate-Starch Hybrid Support: Biocatalytic Properties and Application in Biodegradation of Phenol Red Dye.

Ani Caroline Weber, Bruno Eduardo da Silva, Sabrina Grando Cordeiro, Guilherme Schwingel Henn, Bruna Costa, Jéssica Samara Herek Dos Santos, Valeriano Antonio Corbellini, Eduardo Miranda Ethur, Lucélia Hoehne

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Article in Applied biochemistry and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

9 authors.

Ani Caroline WeberPostgraduate Program in Biotechnology, University of Vale do Taquari - Univates, Av. Avelino Talini, 171, Lajeado, RS, ZIP CODE 95914-014, Brazil.

Bruno Eduardo da SilvaPostgraduate Program in Biotechnology, University of Vale do Taquari - Univates, Av. Avelino Talini, 171, Lajeado, RS, ZIP CODE 95914-014, Brazil.

Sabrina Grando CordeiroPostgraduate Program in Biotechnology, University of Vale do Taquari - Univates, Av. Avelino Talini, 171, Lajeado, RS, ZIP CODE 95914-014, Brazil.

Guilherme Schwingel HennPostgraduate Program in Biotechnology, University of Vale do Taquari - Univates, Av. Avelino Talini, 171, Lajeado, RS, ZIP CODE 95914-014, Brazil.

Bruna CostaPostgraduate Program in Biotechnology, University of Vale do Taquari - Univates, Av. Avelino Talini, 171, Lajeado, RS, ZIP CODE 95914-014, Brazil.

Jéssica Samara Herek Dos SantosPostgraduate Program in Biotechnology, University of Vale do Taquari - Univates, Av. Avelino Talini, 171, Lajeado, RS, ZIP CODE 95914-014, Brazil.

Valeriano Antonio CorbelliniDepartment of Physics and Chemistry, University of Santa Cruz Do Sul - UNISC, Santa Cruz do Sul, RS, Brazil.

Eduardo Miranda EthurPostgraduate Program in Biotechnology, University of Vale do Taquari - Univates, Av. Avelino Talini, 171, Lajeado, RS, ZIP CODE 95914-014, Brazil.

Lucélia HoehnePostgraduate Program in Biotechnology, University of Vale do Taquari - Univates, Av. Avelino Talini, 171, Lajeado, RS, ZIP CODE 95914-014, Brazil. luceliah@univates.br.ORCID http://orcid.org/0000-0002-3221-7007

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001

6 · The paper itself

Abstract

In this study, horseradish peroxidase was extracted, purified, and immobilized on a calcium alginate-starch hybrid support by covalent bonding and entrapment. The immobilized HRP was used for the biodegradation of phenol red dye. A 3.74-fold purification was observed after precipitation with ammonium sulfate and dialysis. An immobilization yield of 88.33%, efficiency of 56.89%, and activity recovery of 50.26% were found. The optimum pH and temperature values for immobilized and free HRP were 5.0 and 50 °C and 6.5 and 60 °C, respectively. The immobilized HRP showed better thermal stability than its free form, resulting in a considerable increase in half-life time (t

Indexed as

AlginatesBiocatalysisBiodegradation, EnvironmentalEnzymes, ImmobilizedHorseradish PeroxidasePhenolsulfonphthaleinStarchTemperatureColoring AgentsEnzyme StabilityGlucuronic AcidHexuronic AcidsHydrogen-Ion ConcentrationAlginatesColoring AgentsEnzymes, ImmobilizedGlucuronic AcidHexuronic AcidsHorseradish PeroxidasePhenolsulfonphthaleinStarchArmoracia rusticanaBiodegradationEnzyme purificationHybrid supportThermostability

Identifiers

PMID37950796

DOI10.3390/molecules25020259

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.