Evidence map›Paper›PMID 37950483›Full record

ArticleJournal of dental research2024

Epigenetic Regulation of Ameloblast Differentiation by HMGN Proteins.

B He, V Kram, T Furusawa, O Duverger, E Y Chu, R Nanduri, M Ishikawa, P Zhang, B A Amendt, J S Lee and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dental research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Genome-Wide Identification and Expression Analysis of theInternational journal of molecular sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

B HeProtein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
V KramMolecular Biology of Bones & Teeth Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
T FurusawaProtein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
O DuvergerCraniofacial Anomalies and Regeneration Section, National Institute of Dental and Craniofacial Research, Bethesda, MD, USA.
E Y ChuDepartment of General Dentistry, Operative Division, University of Maryland, School of Dentistry, Baltimore, MD, USA.
R NanduriProtein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
M IshikawaDepartment of Pathology and Laboratory Medicine and Department of Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.
P ZhangMolecular Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.
B A AmendtDepartment of Anatomy and Cell Biology, and the Craniofacial Anomalies Research Center, Carver College of Medicine, the University of Iowa, Iowa City, IA, USA.
J S LeeCraniofacial Anomalies and Regeneration Section, National Institute of Dental and Craniofacial Research, Bethesda, MD, USA.
M BustinProtein Section, Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0002-5147-7242
National Institutes of Health · USNational Institute of Dental and Craniofacial Research · USUniversity of Iowa · USUniversity of Maryland, Baltimore · USUniversity of Pennsylvania · US

Funding

Co-opting Lef-1 and miR-26b activities to regulate dental stem cells and their progenyR01DE028527 · NIDCR · UNIVERSITY OF IOWA · PI AMENDT, BRAD A, CAO, HUOJUN · 2020 to 2024
$2.1M
6 · The paper itself

Abstract

Dental enamel formation is coordinated by ameloblast differentiation, production of enamel matrix proteins, and crystal growth. The factors regulating ameloblast differentiation are not fully understood. Here we show that the high mobility group N (HMGN) nucleosomal binding proteins modulate the rate of ameloblast differentiation and enamel formation. We found that HMGN1 and HMGN2 proteins are downregulated during mouse ameloblast differentiation. Genetically altered mice lacking HMGN1 and HMGN2 proteins show faster ameloblast differentiation and a higher rate of enamel deposition in mice molars and incisors. In vitro differentiation of induced pluripotent stem cells to dental epithelium cells showed that HMGN proteins modulate the expression and chromatin accessibility of ameloblast-specific genes and affect the binding of transcription factors epiprofin and PITX2 to ameloblast-specific genes. Our results suggest that HMGN proteins regulate ameloblast differentiation and enamel mineralization by modulating lineage-specific chromatin accessibility and transcription factor binding to ameloblast regulatory sites.

Indexed as

Dental Enamel ProteinsHMGN1 ProteinHMGN2 ProteinAmeloblastsAmelogeninAnimalsCell DifferentiationChromatinEpigenesis, GeneticHMGN ProteinsMiceTranscription FactorsAmelogeninChromatinDental Enamel ProteinsHMGN1 ProteinHMGN2 ProteinHMGN ProteinsTranscription Factorsamelogenindevelopmental biologyenamel biomineralization/formationgenomicsmolecular biologytranscription factor(s)

Identifiers

PMID37950483
PMCPMC10850876
OpenAlexW4388596327

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.