Evidence map›Paper›PMID 37950159›Full record

ArticleBMC plant biology2023

Expression of OsHARBI1-1 enhances the tolerance of Arabidopsis thaliana to cadmium.

Nan Jiang, Yang Shi, Mingyu Li, Zhiye Du, Ji Chen, Wenjun Jiang, Yanyan Huang, Min Zhong, Ju Yang, Binhua Hu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
5.2field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 13 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Nan Jiang *College of Ecology and Environment, Chengdu University of Technology, Chengdu, 610059, China.
Yang Shi *College of Ecology and Environment, Chengdu University of Technology, Chengdu, 610059, China.
Mingyu LiCollege of Ecology and Environment, Chengdu University of Technology, Chengdu, 610059, China.
Zhiye DuCollege of Ecology and Environment, Chengdu University of Technology, Chengdu, 610059, China.
Ji ChenCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, China.
Wenjun JiangCollege of Ecology and Environment, Chengdu University of Technology, Chengdu, 610059, China.
Yanyan HuangState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu, 611130, China.
Min ZhongCollege of Ecology and Environment, Chengdu University of Technology, Chengdu, 610059, China.
Ju YangCollege of Ecology and Environment, Chengdu University of Technology, Chengdu, 610059, China.
Binhua HuInstitute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China.
Jin HuangCollege of Ecology and Environment, Chengdu University of Technology, Chengdu, 610059, China. huangjin18@cdut.edu.cn.
Chengdu University of Technology · CNSichuan Agricultural University · CNSichuan Academy of Agricultural Sciences · CN

Funding

National Natural Science Foundation of China 31870383
6 · The paper itself

Abstract

backgroundAs one of the major food crops in the world, rice is vulnerable to cadmium (Cd) pollution. Understanding of the molecular mechanisms of Cd uptake, transport and detoxification in rice is essential for the breeding of low-Cd rice. However, the molecular mechanisms underlying the response of rice to Cd stress remains to be further clarified.

resultsIn this study, a novel Cd-responsive gene OsHARBI1-1 was identified in the rice genome and its expression pattern and function were characterized. Bioinformatics analysis showed that the promoter region of OsHARBI1-1 had multiple cis-acting elements in response to phytohormones and stress, and the expression of OsHARBI1-1 was induced by phytohormones. OsHARBI1-1 protein was targeted to the nucleus. qRT-PCR analysis results showed that the expression of OsHARBI1-1 in the roots was repressed while the expression in the shoots was increased under Cd stress. Heterologous expression of OsHARBI1-1 in yeast conferred tolerance to Cd and reduced Cd content in the cells. Meanwhile, the expression of OsHARBI1-1 in Arabidopsis thaliana (A. thaliana) enhanced the tolerance of A. thaliana to Cd stress. In addition, compared with the wild type plants, the POD activity of transgenic plants was increased, while the SOD and CAT activities were decreased. Interestingly, the accumulation of Cd in the roots of A. thaliana expressing OsHARBI1-1 was significantly increased, whereas the Cd accumulation in the shoots was slightly decreased. Compared to the WT plants, the expression of genes related to Cd absorption and chelation was upregulated in transgenic A. thaliana under Cd stress, while the expression of genes responsible for the translocation of Cd from the roots to the shoots was downregulated. Moreover, the expression of phytohormone-related genes was significantly influenced by the expression of OsHARBI1-1 with and without Cd treatment.

conclusionsFindings of this study suggest that OsHARBI1-1 might play a role in the response of plants to Cd response by affecting antioxidant enzyme activities, Cd chelation, absorption and transport, and phytohormone homeostasis and signaling.

Indexed as

ArabidopsisArabidopsis ProteinsAntioxidantsCadmiumPlant BreedingPlant Growth RegulatorsPlant RootsAntioxidantsArabidopsis ProteinsCadmiumPlant Growth RegulatorsAntioxidant enzyme activitiesArabidopsis thalianaCadmium toleranceHARBI1RiceYeast

Identifiers

PMID37950159
PMCPMC10638780
OpenAlexW4388582889

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.