ArticleCell2023
Proteostasis governs differential temperature sensitivity across embryonic cell types.
Article in Cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Enabling molecular signaling with temperature and ionic-strength independence or programmable dependence.Nature communications · 2026Article
- The alx gene family confers segmental identity to frontonasal cranial neural crest cells.Nature communications · 2026Article
- Embryo-scale Visual Cell Sorting reveals a conserved transcriptomic signature of nucleolar size linked to proteostasis.bioRxiv : the preprint server for biology · 2026Article
- Elevated temperature fatally disrupts nuclear divisions in the early Drosophila embryo.Nature communications · 2026Article
- MIC-Drop-seq: scalable single-cell phenotyping of mutant vertebrate embryos.Nature communications · 2026Article
- Cell type-independent timekeeping gene modules enable embryonic stage prediction in zebrafish.bioRxiv : the preprint server for biology · 2025Article
- The Second International Symposium on Women in Tunicate Biology.Genesis (New York, N.Y. : 2000) · 2025Article
- Article
- A statistical framework for inferring genetic requirements from embryo-scale single-cell sequencing experiments.bioRxiv : the preprint server for biology · 2025Article
- An Improved, High-Yield Method for Isolating Nuclei from Individual Zebrafish Embryos for Single-Nucleus RNA Sequencing.Zebrafish · 2025Article
- Gene module reconstruction identifies cellular differentiation processes and the regulatory logic of specialized secretion in zebrafish.Developmental cell · 2025Article
- Impact of developmental temperature on neural growth, connectivity, and function.Science advances · 2025Article
- Polystyrene nanoplastics impact the bioenergetics of developing zebrafish and limit molecular and physiological adaptive responses to acute temperature stress.The Science of the total environment · 2025Article
- Article
- Understanding genetic variants in context.eLife · 2024Article
- Heterogeneous folding landscapes and predetermined breaking points within a protein family.Protein science : a publication of the Protein Society · 2024Article
- A multiplexed, single-cell sequencing screen identifies compounds that increase neurogenic reprogramming of murine Muller glia.bioRxiv : the preprint server for biology · 2024Article
- Gene module reconstruction elucidates cellular differentiation processes and the regulatory logic of specialized secretion.bioRxiv : the preprint server for biology · 2023Article
- Single-cell analysis of shared signatures and transcriptional diversity during zebrafish development.Developmental cell · 2023Article
- Single-cell sequencing of individual retinal organoids reveals determinants of cell-fate heterogeneity.Cell reports methods · 2023Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
Embryonic development is remarkably robust, but temperature stress can degrade its ability to generate animals with invariant anatomy. Phenotypes associated with environmental stress suggest that some cell types are more sensitive to stress than others, but the basis of this sensitivity is unknown. Here, we characterize hundreds of individual zebrafish embryos under temperature stress using whole-animal single-cell RNA sequencing (RNA-seq) to identify cell types and molecular programs driving phenotypic variability. We find that temperature perturbs the normal proportions and gene expression programs of numerous cell types and also introduces asynchrony in developmental timing. The notochord is particularly sensitive to temperature, which we map to a specialized cell type: sheath cells. These cells accumulate misfolded protein at elevated temperature, leading to a cascading structural failure of the notochord and anatomic defects. Our study demonstrates that whole-animal single-cell RNA-seq can identify mechanisms for developmental robustness and pinpoint cell types that constitute key failure points.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.