ArticleACS synthetic biology2023
Automated Platform for the Plasmid Construction Process.
Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Who cites it
13 citing papers in PubMed.
- Accelerating natural product discovery, characterization and engineering by biofoundries.Natural product reports · 2026Review
- Vision and Development of a Design, Implementation, and Verification Automation (DIVA) Software Platform for DNA Construction.ACS synthetic biology · 2026Article
- PYEAST - A Computational Toolkit for Saccharomyces cerevisiae Genetic Engineering.NPJ systems biology and applications · 2026Article
- Slowpoke: An Automated Golden Gate Cloning Workflow for Opentrons OT-2 and Flex.ACS synthetic biology · 2026Article
- Expanding the genetic toolset: using serine recombinases to integrate riboregulatory elements into industrially relevant microbial chassis.Journal of industrial microbiology & biotechnology · 2026Article
- High-throughput yeast engineering in biofoundries: towards autonomous and scalable synthetic biology.FEMS yeast research · 2026Review
- CloneCoordinate: Open-Source Software for Collaborative DNA Construction.ACS synthetic biology · 2025Article
- On the Choice of the Right Plasmid Vector(s) in the Times of Synthetic Biology.Microbial biotechnology · 2025Review
- The knowledge driven DBTL cycle provides mechanistic insights while optimising dopamine production in Escherichia coli.Microbial cell factories · 2025Article
- Directed Evolution of a Macrolide-Sensing Transcription Factor Biosensor for the Detection of Macrolactone Aglycones via "Effector Walking" and Efflux Pump Deletion.Biochemistry · 2025Article
- Advances in stress-tolerance elements for microbial cell factories.Synthetic and systems biotechnology · 2024Review
- Automated in vivo enzyme engineering accelerates biocatalyst optimization.Nature communications · 2024Review
- Application of valencene and prospects for its production in engineered microorganisms.Frontiers in microbiology · 2024Review
Corrections and comments
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Authors and funding
21 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a growing need for applications capable of handling large synthesis biology experiments. At the core of synthetic biology is the process of cloning and manipulating DNA as plasmids. Here, we report the development of an application named DNAda capable of writing automation instructions for any given DNA construct design generated by the J5 DNA assembly program. We also describe the automation pipeline and several useful features. The pipeline is particularly useful for the construction of combinatorial DNA assemblies. Furthermore, we demonstrate the platform by constructing a library of polyketide synthase parts, which includes 120 plasmids ranging in size from 7 to 14 kb from 4 to 7 DNA fragments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.