ArticlePLoS pathogens2023
Hexadecanamide alleviates Staphylococcus aureus-induced mastitis in mice by inhibiting inflammatory responses and restoring blood-milk barrier integrity.
Article in PLoS pathogens, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
- Artemisia argyi essential oil modulates gut microbiota to influence serum metabolism in rabbits: effects on growth, meat quality, and organ index.Microbiology spectrum · 2026Article
- Protective effect of snail secretion filtrate in an in vitro model of mastitis.Veterinary record open · 2026Article
- Echinatin attenuates LPS-induced mastitis by suppressing IL-17RA and MAPK/NF-κB signaling.BMC veterinary research · 2026Article
- Deep subsurface rock-hosted chemolithotrophic bacterial communities exhibited differential COScientific reports · 2026Article
- UPLC-qToF-MS/MS analysis and anti-inflammatory activities of the soft coralRSC advances · 2026Article
- Hexadecanamide alleviates experimental colitis in mice and modifies the gut microbiome.BMC biology · 2026Article
- Machine Learning-Based Identification of Serum Metabolic Signatures in Adult Patients with Type 1 Diabetes.Diabetes, metabolic syndrome and obesity : targets and therapy · 2026Article
- Ruminal dysbiosis-induced mastitis: new insight into the pathogenesis of mastitis.Journal of animal science and biotechnology · 2025Review
- Article
- Puerarin attenuatesMicrobiology spectrum · 2025Article
- Anthocyanins Prevent Mastitis Exacerbation by Inhibiting PANoptosis Activation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Network Pharmacology and Molecular Docking: Exploring the Mechanism of Peppermint in Mastitis Prevention and Treatment in Dairy Cows.Veterinary sciences · 2025Article
- Gut microbial metabolite indole-3-propionic acid inhibits inflammation and restores blood-milk barrier inFrontiers in microbiology · 2025Article
- Traditional Chinese medicine and plant-derived bioactive compounds as sustainable alternatives to antibiotics in bovine mastitis: a review.Frontiers in veterinary science · 2025Review
- Integrated plasma metabolomic and proteomic analysis uncover the effects and mechanisms of isotretinoin in severe acne.Frontiers in pharmacology · 2025Article
- Ferroptosis in human reproductive tract infections and associated disorders: mechanisms and emerging therapeutic opportunities.Frontiers in immunology · 2025Review
- The dual role of the gut microbiota in breast cancer: from pathogenic mechanisms to emerging therapeutic.Frontiers in microbiology · 2025Review
- Short-Term Exposure of Bisphenol A Deteriorates the Quality of Rabbit Milk by Impairing Milk Fat Synthesis.Food science & nutrition · 2024Article
- Exploring northeast India's culturable soil Actinomycetia for potent antibacterial agents against gram-positive bacterial pathogens of clinical importance.Scientific reports · 2024Article
- Generation of Anti-Mastitis Gene-Edited Dairy Goats with Enhancing Lysozyme Expression by Inflammatory Regulatory Sequence using ISDra2-TnpB System.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Subacute ruminal acidosis (SARA) has been demonstrated to promote the development of mastitis, one of the most serious diseases in dairy farming worldwide, but the underlying mechanism is unclear. Using untargeted metabolomics, we found hexadecanamide (HEX) was significantly reduced in rumen fluid and milk from cows with SARA-associated mastitis. Herein, we aimed to assess the protective role of HEX in Staphylococcus aureus (S. aureus)- and SARA-induced mastitis and the underlying mechanism. We showed that HEX ameliorated S. aureus-induced mastitis in mice, which was related to the suppression of mammary inflammatory responses and repair of the blood-milk barrier. In vitro, HEX depressed S. aureus-induced activation of the NF-κB pathway and improved barrier integrity in mouse mammary epithelial cells (MMECs). In detail, HEX activated PPARα, which upregulated SIRT1 and subsequently inhibited NF-κB activation and inflammatory responses. In addition, ruminal microbiota transplantation from SARA cows (S-RMT) caused mastitis and aggravated S. aureus-induced mastitis, while these changes were reversed by HEX. Our findings indicate that HEX effectively attenuates S. aureus- and SARA-induced mastitis by limiting inflammation and repairing barrier integrity, ultimately highlighting the important role of host or microbiota metabolism in the pathogenesis of mastitis and providing a potential strategy for mastitis prevention.
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