Evidence map›Paper›PMID 37948187›Full record

ArticleSTAR protocols2023

A pipeline for STED super-resolution imaging and Imaris analysis of nanoscale synapse organization in mouse cortical brain slices.

Ezra Kruzich, Rhushikesh A Phadke, Alison Brack, Dimitri Stroumbakis, Oriannys Infante, Alberto Cruz-Martín

Abstract read
In one paragraph

Article in STAR protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Ultrastructural membrane dynamics of mouse and human cortical synapses.bioRxiv : the preprint server for biology · 2025
    Article
  5. Ultrafast endocytosis in mouse cortical inhibitory synapses.bioRxiv : the preprint server for biology · 2025
    Article
  6. Article
  7. Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ezra KruzichNeurobiology Section in the Department of Biology, Boston University, Boston, MA 02215, USA. Electronic address: ezrak@bu.edu.
Rhushikesh A PhadkeMolecular Biology, Cell Biology, and Biochemistry Section in the Department of Biology, Boston University, Boston, MA 02215, USA.
Alison BrackMolecular Biology, Cell Biology, and Biochemistry Section in the Department of Biology, Boston University, Boston, MA 02215, USA.
Dimitri StroumbakisNeurobiology Section in the Department of Biology, Boston University, Boston, MA 02215, USA.
Oriannys InfanteMontclair State University, Montclair, NJ 07043, USA; Summer Undergraduate Research Fellowship Program, Boston University, Boston, MA 02215, USA.
Alberto Cruz-MartínNeurobiology Section in the Department of Biology, Boston University, Boston, MA 02215, USA; Molecular Biology, Cell Biology, and Biochemistry Section in the Department of Biology, Boston University, Boston, MA 02215, USA. Electronic address: martini.cruz@gmail.com.

Funding

Functional heterogeneity of vasoactive intestinal peptide-expressing interneurons in the anterior cingulate cortex R01MH129732 · NIMH · UNIVERSITY OF COLORADO DENVER · PI Alberto Cruz-Martin · 2022 to 2026
$2.0M
NIMH NIH HHS R01 MH129732
6 · The paper itself

Abstract

Advances in super-resolution imaging enable us to delve into its intricate structural and functional complexities with unprecedented detail. Here, we present a pipeline to visualize and analyze the nanoscale organization of cortical layer 1 apical dendritic spines in the mouse prefrontal cortex. We describe steps for brain slice preparation, immunostaining, stimulated emission depletion super-resolution microscopy, and data analysis using the Imaris software package. This protocol allows the study of physiologically relevant brain circuits implicated in neuropsychiatric disorders.

Indexed as

DendritesMicroscopyAnimalsBrainCentral Nervous SystemMiceSynapsesCell BiologyMicroscopyNeuroscience

Identifiers

PMID37948187
PMCPMC10658395

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.