ArticleThe Journal of cell biology2024
Membrane contact site detection (MCS-DETECT) reveals dual control of rough mitochondria-ER contacts.
Article in The Journal of cell biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 21 citations in OpenAlex.
- Advanced imaging of membrane contact sites.Nature cell biology · 2026Review
- Advancing the monitoring of organelle contact sites in vitro and in vivo.Biochemical Society transactions · 2026Review
- Ribosome-binding protein 1: A multidimensional regulator of cancer progression and a novel target for precision therapy (Review).Oncology letters · 2026Review
- Ribosome-binding protein 1 maintains peroxisome biogenesis.Journal of cell science · 2025Article
- A guide to characterizing the dynamic mitochondria-endoplasmic reticulum contact sites.The FEBS journal · 2025Review
- Tom70-mediated mitochondria-nuclear envelope contacts regulate nuclear pore complex inheritance during gametogenesis.bioRxiv : the preprint server for biology · 2025Article
- Nondisruptive inducible labeling of ER-membrane contact sites using the Lamin B receptor.PLoS biology · 2025Article
- Biophysical characterization of anion channels in mitochondrion-endoplasmic-reticulum contact sites.Biophysical journal · 2025Article
- Closing the multichannel gap through computational reconstruction of interaction in super-resolution microscopy.Patterns (New York, N.Y.) · 2025Review
- Rickettsia parkeri forms extensive, stable contacts with the rough endoplasmic reticulum.The Journal of cell biology · 2025Article
- Sex-dependent adaptations in heart mitochondria from transgenic mice overexpressing cytochrome bMitochondrion · 2025Article
- The correlation between mitochondria-associated endoplasmic reticulum membranes (MAMs) and CaActa pharmacologica Sinica · 2025Review
- Cytochrome bAging cell · 2024Article
- Imaging interorganelle contacts at a glance.Journal of cell science · 2024Review
- AI analysis of super-resolution microscopy: Biological discovery in the absence of ground truth.The Journal of cell biology · 2024Review
- Viral regulation of organelle membrane contact sites.PLoS biology · 2024Article
- Imaging and proteomics toolkits for studying organelle contact sites.Frontiers in cell and developmental biology · 2024Review
- Super-resolution microscopy to study membrane nanodomains and transport mechanisms in the plasma membrane.Frontiers in molecular biosciences · 2024Review
- Comparative Analysis of SPLICS and MCS-DETECT for Detecting Mitochondria-ER Contact Sites (MERCs).Contact (Thousand Oaks (Ventura County, Calif.))Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 2 countries.
Funding
Abstract
Identification and morphological analysis of mitochondria-ER contacts (MERCs) by fluorescent microscopy is limited by subpixel resolution interorganelle distances. Here, the membrane contact site (MCS) detection algorithm, MCS-DETECT, reconstructs subpixel resolution MERCs from 3D super-resolution image volumes. MCS-DETECT shows that elongated ribosome-studded riboMERCs, present in HT-1080 but not COS-7 cells, are morphologically distinct from smaller smooth contacts and larger contacts induced by mitochondria-ER linker expression in COS-7 cells. RiboMERC formation is associated with increased mitochondrial potential, reduced in Gp78 knockout HT-1080 cells and induced by Gp78 ubiquitin ligase activity in COS-7 and HeLa cells. Knockdown of riboMERC tether RRBP1 eliminates riboMERCs in both wild-type and Gp78 knockout HT-1080 cells. By MCS-DETECT, Gp78-dependent riboMERCs present complex tubular shapes that intercalate between and contact multiple mitochondria. MCS-DETECT of 3D whole-cell super-resolution image volumes, therefore, identifies novel dual control of tubular riboMERCs, whose formation is dependent on RRBP1 and size modulated by Gp78 E3 ubiquitin ligase activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.