ArticleAnimal bioscience2024
Rumen fermentation and microbial diversity of sheep fed a high-concentrate diet supplemented with hydroethanolic extract of walnut green husks.
Article in Animal bioscience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Effects ofAnimals : an open access journal from MDPI · 2026Article
- Ruminal Fermentation, Methanogenic Archaeal Diversity, and Metabolomic Responses toMetabolites · 2026Article
- Bacillus-based probiotic supplementation reshapes rumen bacterial and fungal communities and enhances carbohydrate-degrading functional capacity in weaned yaks.BMC microbiology · 2026Article
- Effect of chemically protected sodium butyrate on growth performance, serum indicators, rumen fermentation parameters, and microbiota of Gangba sheep.Animal bioscience · 2026Article
- Effect of different levels of Fenugreek sprouts on rumen microbiota and milk yield in Hassani goats.Scientific reports · 2026Article
- Rumen Metagenomic and Muscle Metabolomic Characterization of Meat Quality in Duolang Sheep at Different Ages.Foods (Basel, Switzerland) · 2026Article
- Effect of sorghum-based diets on the abundance and diversity of ruminal methanogenic archaea in Mashona goats.Frontiers in microbiology · 2026Article
- Effect of Flavonoid Sources on Nutrient Intake and Digestibility, Rumen Fermentation, Growth Performance, Milk Production and Endoparasites in Ruminant Production: A Review.TheScientificWorldJournal · 2026Review
- Lyophilized rumen fluid from goats fed different roughage-based diets: fermentation characteristics, microbial communities, and post-reconstitutionFrontiers in microbiology · 2026Article
- Article
- Reproductive performance, digestibility, and rumen bacteria of goats fed two levels of phytogenic mixture.AMB Express · 2025Article
- Host Genetic Effects and Phenotypic Landscapes of Rumen Bacterial Enterotypes in a Large Sheep Population.Animals : an open access journal from MDPI · 2025Article
- Effects of Fermentation and Enzymatic Hydrolysis of Cottonseed Protein on Rumen Fermentation Characteristics, Intestinal Barrier Function, and Hepatic Metabolism in Suckling Lambs.Animals : an open access journal from MDPI · 2025Article
- Capsaicin Modulates Ruminal Fermentation and Bacterial Communities in Beef Cattle with High-Grain Diet-Induced Subacute Ruminal Acidosis.Microorganisms · 2025Article
- Comparative analysis of cecal microbiota and metabolites in relation to growth performance of Tibetan and Hu sheep.Frontiers in microbiology · 2025Article
- Relationships between rumen methanogens and fungal communities and their response to changes in alfalfa forms and starch in sheep diets.Frontiers in microbiomes · 2025Article
- Evaluation of the growth performance, meat quality, and gut microbiota of broilers fed diets containing walnut green husk extract.Poultry science · 2024Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
objectiveThis study aimed to assess the impact of a hydroethanolic extract of walnut green husks (WGH) on rumen fermentation and the diversity of bacteria, methanogenic archaea, and fungi in sheep fed a high-concentrate diet.
methodsFive healthy small-tailed Han ewes with permanent rumen fistula were selected and housed in individual pens. This study adopted a self-controlled and crossover design with a control period and an experimental period. During the control period, the animals were fed a basal diet (with a ratio of concentrate to roughage of 65:35), while during the treatment period, the animals were fed the basal diet supplemented with 0.5% hydroethanolic extract of WGH. Fermentation parameters, digestive enzyme activities, and microbial diversity in rumen fluid were analyzed.
resultsSupplementation of hydroethanolic extract of WGH had no significant effect on feed intake, concentrations of total volatile fatty acids, isovalerate, ammonia nitrogen, and microbial protein (p>0.05). However, the ruminal pH, concentrations of acetate, butyrate and isobutyrate, the ratio of acetate to propionate, protozoa count, and the activities of filter paper cellulase and cellobiase were significantly increased (p<0.05), while concentrations of propionate and valerate were significantly decreased (p<0.05). Moreover, 16S rRNA gene sequencing revealed that the relative abundance of rumen bacteria Christensenellaceae R7 group, Saccharofermentans, and Ruminococcaceae NK4A214 group were significantly increased, while Ruminococcus gauvreauii group, Prevotella 7 were significantly decreased (p<0.05). The relative abundance of the fungus Pseudomonas significantly increased, while Basidiomycota, Fusarium, and Alternaria significantly decreased (p<0.05). However, there was no significant change in the community structure of methanogenic archaea.
conclusionSupplementation of hydroethanolic extract of WGH to a high-concentrate diet improved the ruminal fermentation, altered the structure of ruminal bacterial and fungal communities, and exhibited beneficial effects in alleviating subacute rumen acidosis of sheep.
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