Evidence map›Paper›PMID 37943451›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

The Nanoliter Osmometer: Thermal Hysteresis Measurement.

Nitsan Pariente, Maya Bar Dolev, Ido Braslavsky

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Inhibition of ice recrystallization with designed twistless helical repeat proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  3. Review
  4. Extended Temperature Range of the Ice-Binding Protein Activity.Langmuir : the ACS journal of surfaces and colloids · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Nitsan ParienteInstitute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Maya Bar DolevInstitute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Ido BraslavskyInstitute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel. ido.braslavsky@mail.huji.ac.il.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The nanoliter osmometer is one of the most common tools in the study of ice-binding proteins (IBPs). It is used not only to measure the thermal hysteresis activity of IBPs but also to explore ice shaping, ice adhesion, and ice growth and melting rates and patterns. The advantage of the nanoliter osmometer for the IBP study and for studying single ice crystals lies in the small sample volume, in the range of nanoliters. Such a small volume enables precise determination and control of the temperature with precision in the range of millidegrees. This chapter describes in detail the process of determination of thermal hysteresis using a nanoliter osmometer operated by a LabVIEW interface. We describe the preparation of suitable capillaries and sample injection, which is a challenging step in the measurement. We then describe the procedure of single crystal formation and the determination of the melting and freezing temperatures. Insights on crucial parameters are emphasized.

Indexed as

Cold TemperatureIceCaspase 1TemperatureCaspase 1IceAntifreeze proteinsCrystal growthIce-binding proteinsIce shapingNanoliter osmometerSingle ice crystalThermal hysteresisThermal hysteresis gap

Identifiers

PMID37943451

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.