ArticleMethods in molecular biology (Clifton, N.J.)2024
Ice Shell Purification of Ice-Active Compounds.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Ice fractionation allows for the selective purification of ice-active compounds from aqueous solutions. A variety of approaches have been developed to allow the interaction of ice with solutions of interest. We describe here a version where a thin layer of ice is formed on the inside of a suitable flask, which is then exposed to solutions containing ice-binding molecules under conditions where the ice shell is allowed to grow slowly. Under these conditions, ice-binding compounds are incorporated into the newly formed ice, and other solutes are accumulated in the remaining liquid fraction.
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