Evidence map›Paper›PMID 37942568›Full record

ArticleFEMS microbiology ecology2023

Impact of wastewater treatment upgrade and nitrogen removal on bacterial communities and their interactions in eutrophic prairie streams.

Nathanael T Bergbusch, Alicia R Wong, Jennifer N Russell, Vanessa J Swarbrick, Claire Freeman, Jordyn Bergsveinson, Christopher K Yost, Simon C Courtenay, Peter R Leavitt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEMS microbiology ecology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Nathanael T BergbuschLimnology Laboratory, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.ORCID 0000-0002-8062-6876
Alicia R WongSchool of Environment, Resources and Sustainability (SERS), University of Waterloo, Waterloo, ON N2L 3G1, Canada.
Jennifer N RussellBiology Department, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.
Vanessa J SwarbrickLimnology Laboratory, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.
Claire FreemanInstitute for Microbial Systems and Society, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.
Jordyn BergsveinsonInstitute for Microbial Systems and Society, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.
Christopher K YostBiology Department, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.
Simon C CourtenayInstitute for Microbial Systems and Society, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.
Peter R LeavittLimnology Laboratory, University of Regina, Regina, Saskatchewan S4S 0A2, Canada.

Funding

Canada Foundation for InnovationCanada Research ChairsNSERCUniversity of Regina
6 · The paper itself

Abstract

Eutrophication can impact bacteria by altering fluxes and processing of nutrients and organic matter. However, relatively little is known of how bacterial communities, diversity, and interactions with phytoplankton might respond to nutrient management. We used 16S rRNA amplicon sequencing to compare bacterial assemblages in the water column upstream (control) and downstream (impact) of a wastewater treatment plant (WWTP) located on a eutrophic prairie stream. Sampling occurred before (2012) and after (2018) the 2016 biological nutrient removal (BNR) upgrade that removed >90% of nitrogen (N, mainly NH4+). Multivariate ordination suggested that effluent-impacted bacterial communities were associated mainly with elevated NH4+ concentrations before the upgrade, whereas those after BNR were characteristic of reference systems (low NO3-, diverse regulation). Genera such as Betaproteobacteria and Rhodocyclacea were abundant at impacted sites in 2012, whereas Flavobacterium and a potential pathogen (Legionella) were common at impacted sites in 2018. Nitrifier bacteria (Nitrospira and Nitrosomonas) were present but rare at all sites in 2012, but recorded only downstream of the WWTP in 2018. Generalized additive models showed that BNR reduced bacterial diversity, with ∼70% of the deviance in diversity explained by hydrology, pH, nutrients, and phytoplankton abundance. Overall, NH4+ removal reduced symptoms of cultural eutrophication in microbe assemblages.

Indexed as

WastewaterWater PurificationBacteriaDenitrificationGrasslandNitrogenPhytoplanktonRNA, Ribosomal, 16SNitrogenRNA, Ribosomal, 16SWastewater16S rRNAammoniabiological nutrient removaleffluentphytoplanktonQu’Appelle River

Identifiers

PMID37942568
PMCPMC10662661

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.