A TaqMan-based RT-qPCR assay for serotyping of Southern African territories (SAT) 2 strains of Foot-and-Mouth disease virus (FMDV) in Southern Africa.

Tshephang I Kabelo, Elliot M Fana, Monamodi Kesamang, Joseph Hyera, Kebaneilwe Lebani

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Article in BMC research notes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 authors.

Tshephang I KabeloDepartment of Biological Sciences and Biotechnology, Faculty of Science, International University of Science and Technology, Private Bag 16, Palapye, Botswana.

Elliot M FanaWOAH (OIE) Reference Laboratory for Foot-and-Mouth Disease, Botswana Vaccine Institute, Private Bag 0031, Gaborone, Botswana.

Monamodi KesamangDepartment of Biological Sciences and Biotechnology, Faculty of Science, International University of Science and Technology, Private Bag 16, Palapye, Botswana.

Joseph HyeraWOAH (OIE) Reference Laboratory for Foot-and-Mouth Disease, Botswana Vaccine Institute, Private Bag 0031, Gaborone, Botswana.

Kebaneilwe LebaniDepartment of Biological Sciences and Biotechnology, Faculty of Science, International University of Science and Technology, Private Bag 16, Palapye, Botswana. lebanik@biust.ac.bw.

Funding

Botswana International University of Science and Technology S00220

6 · The paper itself

Abstract

objectiveDetermining the serotype of circulating virus strains is important in implementing effective vaccination. In this study, Foot-and-Mouth Disease (FMD) Southern African territory 2 (SAT2) specific primers and TaqMan probe were designed towards rapid SAT2 detection and serotyping. The primers were tested by endpoint reverse transcription (RT) polymerase chain reaction (PCR) and quantitative PCR (RT-qPCR) using the vaccine strain SAT2035. The SAT2 serotype-specific RT-qPCR assay was compared with currently used ELISA and VP1 sequencing using Cohen's kappa statistics.

resultsThe primers yielded amplicons of band size 190 bp during endpoint RT-PCR. When coupled with the probe, the primers reaction efficiency was determined to be 99% with an r

Indexed as

Foot-and-Mouth DiseaseFoot-and-Mouth Disease VirusAfrica, SouthernAnimalsSerogroupSerotypingFMDVRT-qPCRSAT2Serotyping

Identifiers

PMID37941022

DOI10.1186/s13104-023-06586-7

PMCPMC10634033

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.