ArticleISME communications2021
Critical evaluation of faecal microbiome preservation using metagenomic analysis.
Article in ISME communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Improving immune-related health outcomes post-cesarean birth with a gut microbiome-based program: A randomized controlled trial.Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology · 2025Trial
- Emerging Strategies for the Preservation of Microorganisms of Biomedical and Environmental Importance.Methods in molecular biology (Clifton, N.J.) · 2027Review
- Metagenomic Insights Into Red Sea Biodiversity Across the Web of Life.Environmental microbiology · 2026Article
- Profiling the Athletes' Gut Microbiome: A Critical Methodological Perspective on 16S Metabarcoding and Shotgun Metagenomics.Biology · 2026Review
- African carnivore gut bacterial diversity and composition are associated with sample condition but not storage technique.Animal microbiome · 2026Article
- Article
- Post-defecation exposure alters gut microbiota of forest musk deer with implications for conservation metagenomics.Applied microbiology and biotechnology · 2026Article
- Article
- Gut microbe's consortium in pregnant women influenced by diet of North-east India: A metagenomic study.Journal of family medicine and primary care · 2025Article
- Comparative study of sample storage conditions on gut dysbiosis in peripheral artery disease.Research square · 2025Article
- Best practices for the experimental design of one health studies on companion animal and owner microbiomes - From data collection to analysis.One health (Amsterdam, Netherlands) · 2025Review
- Generation Victoria (GenV): protocol for a longitudinal birth cohort of Victorian children and their parents.BMC public health · 2025Article
- Evaluating long-term stool preservation methods for maximizing the recovery of viable human fecal microbiota.Gut microbes reports · 2025Article
- Article
- Temporal patterns of gut microbiota in lemurs (Eulemur rubriventer) living in intact and disturbed habitats in a novel sample type.American journal of primatology · 2025Article
- Omnigene-GutAMB Express · 2024Article
- Methodology for Biological Sample Collection, Processing, and Storage in the Newcastle 1000 Pregnancy Cohort: Protocol for a Longitudinal, Prospective Population-Based Study in Australia.JMIR research protocols · 2024Article
- Multi-Omics Analysis Unravels the Impact of Stool Sample Logistics on Metabolites and Microbial Composition.Microorganisms · 2024Article
- Effects of Defatted Rice Bran-Fortified Bread on the Gut Microbiota Composition of Healthy Adults With Low Dietary Fiber Intake: Protocol for a Crossover Randomized Controlled Trial.JMIR research protocols · 2024Article
- Delivery mode is a larger determinant of infant gut microbiome composition at 6 weeks than exposure to peripartum antibiotics.Microbial genomics · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to preserve microbial communities in faecal samples is essential as increasing numbers of studies seek to use the gut microbiome to identify biomarkers of disease. Here we use shotgun metagenomics to rigorously evaluate the technical and compositional reproducibility of five room temperature (RT) microbial stabilisation methods compared to the best practice of flash-freezing. These methods included RNALater, OMNIGene-GUT, a dry BBL swab, LifeGuard, and a novel method for preserving faecal samples, a Copan FLOQSwab in an active drying tube (FLOQSwab-ADT). Each method was assessed using six replicate faecal samples from five participants, totalling 180 samples. The FLOQSwab-ADT performed best for both technical and compositional reproducibility, followed by RNAlater and OMNIgene-GUT. LifeGuard and the BBL swab had unpredictable outgrowth of Escherichia species in at least one replicate from each participant. We further evaluated the FLOQSwab-ADT in an additional 239 samples across 10 individuals after storage at -20 °C, RT, and 50 °C for four weeks compared to fresh controls. The FLOQSwab-ADT maintained its performance across all temperatures, indicating this method is an excellent alternative to existing RT stabilisation methods.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.